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Bethyl Laboratories
PTRF Polyclonal Antibody
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FIGURE: 1 / 1
PTRF Antibody (A301-269A) in WB
Detection of human PTRF by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PTRF antibody (Product # A301-269A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. PTRF was also immunoprecipitated by rabbit anti-PTRF antibodies A301-270A and A301-271A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
Product Details
A301-269A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 125 and 175 of Human RNA polymerase I and transcript release factor.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The polymerase I and transcript release factor (PTRF) enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts, regulating rRNA transcription by promoting the dissociation of transcription complexes and the re-initiation of polymerase I on nascent rRNA transcripts. PTRF also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins, translocating from caveolae to the cytoplasm after insulin stimulation. PTRF is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Caveolae-associated protein 1; Cavin-1; congenital generalized lipodystrophy 4; FLJ90031; Polymerase I and transcript release factor; RNA polymerase I and transcript release factor; TTF-I interacting peptide 12; unnamed protein product
Gene Aliases: CAVIN; cavin-1; CAVIN1; CGL4; FKSG13; PTRF
UniProt ID: (Human) Q6NZI2
Entrez Gene ID: (Human) 284119
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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