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Bethyl Laboratories

NHERF1 Polyclonal Antibody

View all (17) EBP50 antibodies

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NHERF1 Polyclonal Antibody

Product Details

A302-974A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunohistochemistry (IHC)

1:100-1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunoprecipitation (IP)

2-5 µg/mg lysate
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 275 and 325 of human Sodium/Hydrogen Exchanger Regulatory Factor 1.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EBP50; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor 1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHE-RF; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; SLC9A3 regulator 1; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; Solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1; unnamed protein product

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Gene Aliases: EBP-50; EBP50; NHE-RF; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1

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UniProt ID: (Human) O14745, (Mouse) P70441

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Entrez Gene ID: (Human) 9368, (Mouse) 26941

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Function(s)
receptor binding protein binding beta-catenin binding gamma-aminobutyric acid transmembrane transporter activity chloride channel regulator activity phosphatase binding PDZ domain binding beta-2 adrenergic receptor binding type 2 metabotropic glutamate receptor binding type 3 metabotropic glutamate receptor binding identical protein binding protein anchor dopamine receptor binding binding, bridging growth factor receptor binding channel activator activity protein domain specific binding protein complex binding protein complex scaffold protein self-association
Process(es)
morphogenesis of an epithelium renal sodium ion transport sodium ion transport plasma membrane organization nuclear migration adenylate cyclase-activating dopamine receptor signaling pathway sensory perception of sound protein localization negative regulation of cell proliferation regulation of cell shape regulation of cell size negative regulation of platelet-derived growth factor receptor signaling pathway fibroblast migration negative regulation of fibroblast migration negative regulation of sodium ion transport Wnt signaling pathway gland morphogenesis microvillus assembly actin cytoskeleton organization cellular phosphate ion homeostasis bile acid secretion glutathione transport cilium organization establishment of epithelial cell apical/basal polarity maintenance of epithelial cell apical/basal polarity regulation of protein kinase activity negative regulation of mitotic cell cycle establishment of localization in cell establishment of Golgi localization negative regulation of protein kinase B signaling gamma-aminobutyric acid import auditory receptor cell stereocilium organization phospholipase C-activating dopamine receptor signaling pathway protein-containing complex assembly renal absorption negative regulation of ERK1 and ERK2 cascade protein localization to plasma membrane negative regulation of canonical Wnt signaling pathway cerebrospinal fluid circulation renal phosphate ion absorption import across plasma membrane transport across blood-brain barrier regulation of renal phosphate excretion negative regulation of cell motility positive regulation of intrinsic apoptotic signaling pathway negative regulation of phosphatidylinositol 3-kinase signaling cAMP-mediated signaling negative regulation of cell migration negative regulation of sodium:proton antiporter activity cellular protein localization regulation of excretion
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