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Bethyl Laboratories
eIF4AIII/EIF4A3 Polyclonal Antibody
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FIGURE: 1 / 3
eIF4AIII/EIF4A3 Antibody (A302-980A) in IHC (P)
Detection of human eIF4AIII/EIF4A3 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 (Product # A302-980A) (Lot 1 left panel) and (Product # A302-981A) (Lot 1 right panel) used at a dilution of 1:5,000 (0.2 µg/mL) and 1:200 (1 µg/mL). Detection: DAB.
Product Details
A302-980A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1 and 50 of human Eukaryotic Translation Initiation Factor 4A3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine and Pig
Target Information
eIF4A3 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. eIF4A3 is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DDX48; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; eIF-4A-III; Eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A, isoform 3; KIAA0111; NMP 265; Nuclear matrix protein 265; unnamed protein product
Gene Aliases: 2400003O03Rik; DDX48; eIF-4A-III; eIF4A-III; EIF4A3; eIF4AIII; Fal1; KIAA0111; mKIAA0111; MUK34; NMP265; NUK34; RCPS
UniProt ID: (Human) P38919, (Mouse) Q91VC3
Entrez Gene ID: (Human) 9775, (Mouse) 192170
nucleotide binding
nucleic acid binding
RNA binding
RNA helicase activity
mRNA binding
helicase activity
protein binding
ATP binding
poly(A) binding
hydrolase activity
ATPase activity
selenocysteine insertion sequence binding
RNA stem-loop binding
ribonucleoprotein complex binding
ATP-dependent RNA helicase activity
poly(A) RNA binding
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
mRNA splicing, via spliceosome
rRNA processing
mRNA processing
mRNA export from nucleus
regulation of translation
associative learning
RNA splicing
negative regulation of gene expression
negative regulation of translation
exploration behavior
positive regulation of translation
embryonic cranial skeleton morphogenesis
mRNA transport
cellular response to selenite ion
negative regulation of excitatory postsynaptic potential
regulation of translation at postsynapse, modulating synaptic transmission
negative regulation of selenocysteine incorporation
cellular response to brain-derived neurotrophic factor stimulus
regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
RNA secondary structure unwinding
positive regulation of transcription from RNA polymerase II promoter
positive regulation of mRNA splicing, via spliceosome
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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