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Bethyl Laboratories
NO66 Polyclonal Antibody
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FIGURE: 1 / 1
NO66 Antibody (A303-199A) in WB
Detection of human NO66 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NO66 antibody (Product # A303-199A) used for WB at 0.1 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 6 µg/mg lysate. NO66 was also immunoprecipitated by rabbit anti-NO66 antibody A303-198A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and ... View More
Product Details
A303-199A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 50 and 100 of human Nucleolar Protein 66.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation. May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 60S ribosomal protein L8 histidine hydroxylase; Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66; FLJ21802; histone lysine demethylase NO66; hsNO66; lysine-specific demethylase NO66; MYC-associated protein with JmjC domain; Nucleolar protein 66; Ribosomal oxygenase 1; Ribosomal oxygenase NO66; ROX; unnamed protein product; up-regulated in lung cancer 2
Gene Aliases: C14orf169; hsNO66; JMJD9; MAPJD; NO66; RIOX1; ROX; URLC2
UniProt ID: (Human) Q9H6W3
Entrez Gene ID: (Human) 79697
iron ion binding
protein binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
histone demethylase activity (H3-K4 specific)
histone demethylase activity (H3-trimethyl-K4 specific)
peptidyl-histidine dioxygenase activity
metal ion binding
dioxygenase activity
histone demethylase activity (H3-K36 specific)
protein demethylase activity
histone H3-di/monomethyl-lysine-36 demethylase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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