Search
Bethyl Laboratories
Alpha-Adducin Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 4
Alpha-Adducin Antibody (A303-714A) in IHC (P)
Detection of human Alpha-Adducin by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Alpha-Adducin (Product # A303-714A) (lot 1) used at a dilution of 1:5,000 (0.2 µg/mL) (right) and (Product # A303-713A) (lot 1) used at a dilution of 1:5,000 (0.2 µg/mL) (left). Detection: DAB.
Product Details
A303-714A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 632 and 682 of human Adducin 1 (alpha).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: ADD2; ADDB; adducin 1 (alpha); Adducin beta; Adducin-1; Adducin-alpha; Adducin-beta; Adducin1; Alpha adducin; Alpha-adducin; alpha-adducin 1; beta Adducin; Beta-adducin; calmodulin-binding protein; erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; unnamed protein product
Gene Aliases: ADD1; ADDA
UniProt ID: (Human) P35611
Entrez Gene ID: (Human) 118
RNA binding
actin binding
protein binding
calmodulin binding
cytoskeletal adaptor activity
spectrin binding
protein homodimerization activity
cadherin binding
protein heterodimerization activity
protein dimerization activity
actin filament binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
cytoskeleton organization
actin cytoskeleton organization
regulation of cellular component size
barbed-end actin filament capping
actin filament bundle assembly
positive regulation of developmental process
positive regulation of cellular component organization
cellular response to calcium ion
positive regulation of establishment of endothelial barrier
positive regulation of adherens junction organization
regulation of multicellular organismal development
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support