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Bethyl Laboratories
Phospho-RNA Polymerase II (Ser5) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-RNA Polymerase II (Ser5) Antibody (A304-408A) in IHC (P)
Detection of human Phospho RNA Polymerase II (S5) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) (Product # A304-408A) (Lot 3) used at a dilution of 1:1000 (0.2 µg/mL). Detection: DAB.
Product Details
A304-408A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phosphorylated synthetic peptide, which represented a portion of Human RNA Polymerase II around serine 5 of the C-terminal repeat YSPTSPS. of Human RNA Polymerase II.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7-8, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with D. melanogaster
Target Information
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3'-5' exoribonuclease; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; MGC75453; Pol II S2p; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II; RNA polymerase II 1; RNA polymerase II C-terminal domain; RNA polymerase II carboxy-terminal domain; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; RPB220; RPO21; SUA8; unnamed protein product
Gene Aliases: 220kDa; hRPB220; hsRPB1; NEDHIB; POLR2; POLR2A; POLRA; RPB1; RPBh1; Rpii215; RpIILS; RPO2; Rpo2-1; RPOL2
UniProt ID: (Human) P24928, (Mouse) P08775
Entrez Gene ID: (Human) 5430, (Mouse) 20020
magnesium ion binding
nucleic acid binding
DNA binding
RNA binding
DNA-directed RNA polymerase activity
RNA-directed RNA polymerase activity
protein binding
zinc ion binding
transferase activity
nucleotidyltransferase activity
hydrolase activity
kinase binding
ubiquitin protein ligase binding
RNA polymerase activity
metal ion binding
microfibril binding
promoter-specific chromatin binding
core promoter binding
RNA polymerase II activity
poly(A) RNA binding
DNA-directed RNA polymerase
transcription, RNA-templated
transcription, DNA-templated
DNA-templated transcription, termination
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
positive regulation of gene expression
positive regulation of RNA splicing
mRNA transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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