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          • Primary Antibodies ›
          • PPAR gamma Antibodies

          Bethyl Laboratories

          PPAR-gamma Polyclonal Antibody

          View all (33) PPAR gamma antibodies

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          Cite PPAR-gamma Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          PPAR-gamma Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          PPAR-gamma Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          PPAR-gamma Antibody (A304-460A) in WB

          Detection of mouse PPAR-gamma by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, differentiated mouse NIH 3T3-L1, and mouse NIH3T3-L1 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPAR-gamma antibody (Product # A304-460A) (lot A304-460A-1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PPAR-gamma Antibody in Western Blot (WB)
          PPAR-gamma Antibody in Western Blot (WB)
          PPAR-gamma Antibody in Immunoprecipitation (IP)
          PPAR-gamma Polyclonal Antibody

          Product Details

          A304-460A

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:10,000-1:25,000
          -

          Immunoprecipitation (IP)

          2-10 µg/mg lysate
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Region between residue 50 to 100 of Human Peroxisome Proliferator-Activated Receptor Gamma.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          tris citrate/phosphate, pH 7-8

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          The recommended shelf life for this product is 1 year from date of receipt.

          Target Information

          This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: HUMPPARG; Nuclear receptor subfamily 1 group C member 3; OTTHUMP00000185032; OTTHUMP00000185036; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; Peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor-gamma 5; peroxisome proliferator-activated receptor-gamma splicing; PPAR gamma; PPAR-gamma; PPARy; unnamed protein product

          View more View less

          Gene Aliases: CIMT1; FPLD3; GLM1; NR1C3; PPAR-gamma; PPAR-gamma2; PPARG; PPARG1; PPARG2; PPARG5; PPARgamma; PPARgamma2

          View more View less

          UniProt ID: (Human) P37231, (Mouse) P37238

          View more View less

          Entrez Gene ID: (Human) 5468, (Mouse) 19016

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription cofactor binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding nucleic acid binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding prostaglandin receptor activity protein binding zinc ion binding enzyme binding peptide binding identical protein binding sequence-specific DNA binding metal ion binding retinoid X receptor binding arachidonic acid binding DBD domain binding LBD domain binding WW domain binding alpha-actinin binding R-SMAD binding E-box binding STAT family protein binding DNA-binding transcription factor binding RNA polymerase II regulatory region DNA binding core promoter sequence-specific DNA binding steroid hormone receptor activity transcription factor binding drug binding protein phosphatase binding estrogen receptor binding ligand-dependent nuclear receptor transcription coactivator activity activating transcription factor binding transcription regulatory region DNA binding C4 zinc finger nuclear receptor DNA-binding transcription factor zinc finger transcription factor gene-specific transcriptional regulator
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter placenta development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter fatty acid metabolic process G-protein coupled receptor signaling pathway response to nutrient regulation of blood pressure hormone-mediated signaling pathway positive regulation of gene expression negative regulation of gene expression negative regulation of macrophage derived foam cell differentiation positive regulation of cholesterol efflux negative regulation of cholesterol storage negative regulation of lipid storage long-chain fatty acid transport negative regulation of angiogenesis cell differentiation monocyte differentiation BMP signaling pathway negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway intracellular receptor signaling pathway epithelial cell differentiation negative regulation of defense response response to nutrient levels positive regulation of cholesterol transport cellular response to insulin stimulus response to lipid peroxisome proliferator activated receptor signaling pathway glucose homeostasis regulation of circadian rhythm mRNA transcription from RNA polymerase II promoter negative regulation of MAPK cascade negative regulation of blood vessel endothelial cell migration innate immune response cell fate commitment positive regulation of fat cell differentiation negative regulation of osteoblast differentiation positive regulation of lipid metabolic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of fatty acid metabolic process positive regulation of transcription from RNA polymerase II promoter retinoic acid receptor signaling pathway cell maturation rhythmic process negative regulation of smooth muscle cell proliferation negative regulation of inflammatory response white fat cell differentiation regulation of transport negative regulation of multicellular organismal process lipid homeostasis negative regulation of interferon-gamma-mediated signaling pathway negative regulation of SMAD protein import into nucleus positive regulation of adiponectin secretion cellular response to low-density lipoprotein particle stimulus cellular response to hypoxia negative regulation of mitochondrial fission positive regulation of lipoprotein transport nuclear receptor-mediated signaling pathway regulation of cellular response to insulin stimulus negative regulation of extracellular matrix assembly negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of cardiac muscle hypertrophy in response to stress negative regulation of cellular response to transforming growth factor beta stimulus positive regulation of adipose tissue development negative regulation of connective tissue replacement involved in inflammatory response wound healing negative regulation of vascular smooth muscle cell proliferation negative regulation of receptor signaling pathway via STAT positive regulation of vascular associated smooth muscle cell apoptotic process negative regulation of vascular endothelial cell proliferation positive regulation of apoptotic signaling pathway negative regulation of cytokine production response to dietary excess diet induced thermogenesis negative regulation of acute inflammatory response transcription, DNA-templated transcription from RNA polymerase II promoter activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response signal transduction negative regulation of cell proliferation response to light stimulus regulation of gene expression negative regulation of receptor biosynthetic process negative regulation of sequestering of triglyceride regulation of lipid metabolic process fatty acid oxidation negative regulation of cell growth response to food response to retinoic acid negative regulation of collagen biosynthetic process lipoprotein transport positive regulation of apoptotic process steroid hormone mediated signaling pathway fat cell differentiation regulation of fat cell differentiation low-density lipoprotein particle receptor biosynthetic process positive regulation of fatty acid oxidation positive regulation of oligodendrocyte differentiation brown fat cell differentiation positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of telomerase activity response to low-density lipoprotein particle positive regulation of phagocytosis, engulfment regulation of transcription involved in cell fate commitment cellular response to lithium ion cellular response to organic cyclic compound negative regulation of peptide hormone secretion negative regulation of cellular response to insulin stimulus negative regulation of pancreatic stellate cell proliferation
          It has to be done as per old AB suggested Products section.

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