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Bethyl Laboratories
NSFL1C Polyclonal Antibody
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FIGURE: 1 / 2
NSFL1C Antibody (A304-565A) in WB
Detection of human and mouse NSFL1C by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NSFL1C antibody (Product # A304-565A) (lot A304-565A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A304-565A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 25 to 75 of human NSFL1 (p97) Cofactor (p47).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine and Orangutan
Target Information
p47, also known as NSFL1C, UBX1, UBXD10 or UBXN2C, is a 370 amino acid protein that localizes to both the nucleus and the golgi apparatus (specifically to golgi stacks) and contains one SEP domain and one UBX domain. Functioning as part of a ternary complex with VCP (a protein involved in the heterotypic fusion of transport vesicles with their target membranes) and Syntaxin 5, p47 interacts with and reduces the ATPase activity of VCP and is required for the fragmentation of golgi stacks during mitosis and for subsequent reassembly of golgi stacks after mitosis. p47 is subject to phosphorylation during mitosis, which inhibits p47-golgi interaction and is, therefore, required for proper golgi stack formation and cisternal regrowth. Human p47 shares 89% sequence identity with its mouse counterpart, suggesting a conserved role between species. Multiple isoforms of p47 exist due to alternative splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NSFL1 (p97) cofactor (p47); NSFL1 cofactor p47; P47; p97 cofactor p47; RP4-776F14.2; SHP1 homolog; UBX domain-containing protein 2C; unnamed protein product
Gene Aliases: dJ776F14.1; Munc-18c; NSFL1C; P47; Stxbp3a; UBX1; UBXD10; UBXN2C
UniProt ID: (Human) Q9UNZ2, (Mouse) Q9CZ44
Entrez Gene ID: (Human) 55968, (Mouse) 386649
autophagosome assembly
establishment of mitotic spindle orientation
ubiquitin-dependent protein catabolic process
Golgi organization
nuclear envelope reassembly
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of mitotic centrosome separation
membrane fusion
negative regulation of protein localization to centrosome
regulation of catalytic activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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