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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Clathrin Heavy Chain Antibodies

          Bethyl Laboratories

          CHC Polyclonal Antibody

          View all (34) Clathrin Heavy Chain antibodies

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          Cite CHC Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          CHC Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          CHC Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          CHC Antibody (A304-743A) in WB

          Detection of human and mouse CHC by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHC antibody (Product # A304-743A) (lot A304-743A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CHC Antibody in Western Blot (WB)
          CHC Antibody in Immunoprecipitation (IP)
          CHC Polyclonal Antibody

          Product Details

          A304-743A

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          -

          Immunoprecipitation (IP)

          2-10 µg/mg lysate
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Region between residue 1625 to 1675 of human Clathrin, Heavy Chain.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          phosphate/tris citrate, pH 7-8

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          The recommended shelf life for this product is 1 year from date of receipt.

          Based on 100% sequence identity, this antibody is predicted to react with Rat and Bovine

          Target Information

          Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain on chromosome 17; Clathrin heavy chain on chromosome 22; clathrin, heavy chain (Hc); clathrin, heavy chain-like 1; clathrin, heavy polypeptide (Hc); Clathrin, heavy polypeptide D; clathrin, heavy polypeptide-like 1; clathrin, heavy polypeptide-like 2; CLH-17; CLH-22

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          Gene Aliases: 3110065L21Rik; CHC; CHC17; CHC22; CLH-17; CLH17; CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD; Hc; KIAA0034; R74732

          View more View less

          UniProt ID: (Human) Q00610, (Human) P53675, (Mouse) Q68FD5

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          Entrez Gene ID: (Human) 1213, (Human) 8218, (Mouse) 67300

          View more View less

          Function(s)
          double-stranded RNA binding structural molecule activity protein binding protein kinase binding clathrin light chain binding poly(A) RNA binding signal transducer activity protein C-terminus binding ankyrin binding heat shock protein binding peptide binding vesicle coat protein
          Process(es)
          osteoblast differentiation intracellular protein transport receptor-mediated endocytosis mitotic nuclear division antigen processing and presentation of exogenous peptide antigen via MHC class II receptor internalization transferrin transport retrograde transport, endosome to Golgi Wnt signaling pathway, planar cell polarity pathway negative regulation of hyaluronan biosynthetic process negative regulation of protein localization to plasma membrane signal transduction anatomical structure morphogenesis positive regulation of glucose import Golgi organization vesicle-mediated transport mitotic spindle assembly
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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