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Bethyl Laboratories
ERH Polyclonal Antibody
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FIGURE: 1 / 1
ERH Antibody (A305-402A) in IP
Detection of human ERH by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERH antibody (Product # A305-402A) (lot 1) used for IP at 6 µg per reaction. ERH was also immunoprecipitated by rabbit anti-ERH antibody BL21382. For blotting immunoprecipitated ERH, A305-402A was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A305-402A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 54 to 104 of human Enhancer of rudimentary homolog.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Zebrafish, Bovine and Pig
Target Information
The Enhancer of Rudimentary Homolog (ERH) is a highly conserved protein with significant roles in the regulation of gene expression and cell cycle control. Structurally, ERH forms homodimers, characterized by a crystal structure resolving at 2.0 A, which reveals its ability to bind specific protein regions via a hydrophobic groove. Functionally, ERH interacts with various cellular components, notably integrating into the Microprocessor complex where it facilitates microRNA maturation by binding with the N-terminus of DGCR8 at a 2:2 stoichiometry. This interaction underscores ERH's role in gene silencing, contributing to the biogenesis of small interfering RNAs (siRNAs). Additionally, ERH is implicated in transcriptional regulation and has been associated with DNA replication, suggesting its involvement in broader genomic functions. Despite its diverse roles, ERH operates in a plug-and-play manner, allowing its interaction with different cellular factors to fulfill pleiotropic functions across metazoans.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Enhancer of rudimentary homolog; ERH; unnamed protein product
Gene Aliases: DROER; ERH
UniProt ID: (Human) P84090
Entrez Gene ID: (Human) 2079
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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