Search
Invitrogen
Goat anti-Human IgG1 (Heavy chain) Recombinant Secondary Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 10
Human IgG1 (Heavy chain) Secondary Antibody (A55733) in ICC/IF
Immunofluorescence analysis was performed on fixed and permeabilized T-47D cells stained with MUC1/ Gatipotuzumab Recombinant Human Monoclonal Antibody (Product # MA5-42157, 1:500 dilution) followed by Goat anti-Human IgG1 (Heavy Chain) Recombinant Secondary Antibody (Product # A55733, 1:500 dilution) and detected using Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 4... View More
Product Details
A55733
Applications
Tested Dilution
Western Blot (WB)
1:1,000
Immunocytochemistry (ICC/IF)
1:500-1:1,000
ELISA (ELISA)
1:1,000
Peptide Array (Array)
1:1000
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Secondary Antibody
Clone
3H60L9
Immunogen
Purified Human IgG
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
250 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Target
IgG1 (Heavy chain)
Antibody Form
Whole Antibody
Product Specific Information
This Goat anti-Human IgG1 (Heavy chain) Recombinant Monoclonal Antibody reacts with human IgG1 heavy chain with no cross-reactivity to human IgG2, IgG3, IgG4, IgA or IgM or various species immunoglobulins, including monkey IgG.
The sensitivity and specificity of each lot is confirmed using ELISA.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support