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          • Primary Antibodies ›
          • Leptin Antibodies

          Invitrogen

          Leptin Monoclonal Antibody (3)

          View all (33) Leptin antibodies

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          Cite Leptin Monoclonal Antibody (3)

          Additional Information:
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          • Antibody Testing Data (2)
          Leptin Antibody in Immunohistochemistry (IHC)
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          Leptin Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Leptin Antibody (ABS 022-03-02) in IHC

          Immunohistochemical analysis of Leptin in malignant carcinoma using a Leptin monoclonal antibody (Product # ABS 022-03-02). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Leptin Antibody in Immunohistochemistry (IHC)
          Leptin Antibody in ELISA (ELISA)
          Leptin Monoclonal Antibody (3)

          Product Details

          ABS 022-03-02

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          Assay-dependent
          -

          ELISA (ELISA)

          1:40,000
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          3

          Immunogen

          Purified, recombinant Leptin.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.09 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          15mM sodium azide

          Storage conditions

          4°C, store in dark

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_667480

          Product Specific Information

          ABS 022-03-02 detects Leptin in human samples.

          ABS 022-03-02 has been successfully used in ELISA and immunohistochemistry procedures. ABS 022-03-02 binds free Leptin in solution. In sandwich ELISA, ABS 022-03-02 works as a capture antibody with ABS 022-11-02 as a detection antibody.

          NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

          Target Information

          Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: CD295 antigen; H-Leptin; HuB219; LEP-R; Leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); ob; OB-R; obese; Obese protein; obese, mouse, homolog of; obesity; Obesity factor; obesity protein; unnamed protein product

          View more View less

          Gene Aliases: LEP; LEPD; OB; OBS

          View more View less

          UniProt ID: (Human) P41159

          View more View less

          Entrez Gene ID: (Human) 3952

          View more View less

          Function(s)
          DNA binding receptor binding hormone activity protein binding peptide hormone receptor binding leptin receptor binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter angiogenesis ovulation from ovarian follicle response to hypoxia positive regulation of cytokine production placenta development regulation of endothelial cell proliferation response to dietary excess cardiac muscle hypertrophy glucose metabolic process regulation of gluconeogenesis energy reserve metabolic process glycerol biosynthetic process lipid metabolic process fatty acid beta-oxidation phagocytosis signal transduction JAK-STAT cascade female pregnancy response to nutrient circadian rhythm cholesterol metabolic process bile acid metabolic process regulation of blood pressure positive regulation of cell proliferation determination of adult lifespan adult feeding behavior fatty acid catabolic process negative regulation of metabolic process negative regulation of autophagy negative regulation of lipid storage response to activity regulation of metabolic process sexual reproduction central nervous system neuron development insulin secretion T cell differentiation regulation of intestinal cholesterol absorption response to nutrient levels positive regulation of TOR signaling negative regulation of appetite prostaglandin secretion response to estradiol positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production regulation of natural killer cell activation regulation of natural killer cell proliferation response to insulin cellular response to insulin stimulus response to vitamin E leptin-mediated signaling pathway positive regulation of luteinizing hormone secretion positive regulation of peroxisome proliferator activated receptor signaling pathway intracellular signal transduction bone mineralization involved in bone maturation aorta development negative regulation of appetite by leptin-mediated signaling pathway positive regulation of T cell proliferation regulation of natural killer cell mediated cytotoxicity positive regulation of protein import into nucleus hormone metabolic process glucose homeostasis eating behavior negative regulation of apoptotic process positive regulation of ion transport positive regulation of MAPK cascade cellular response to leptin stimulus response to ethanol regulation of fat cell differentiation regulation of angiogenesis negative regulation of vasoconstriction negative regulation of glucose import positive regulation of JAK-STAT cascade positive regulation of insulin receptor signaling pathway regulation of bone remodeling positive regulation of follicle-stimulating hormone secretion regulation of lipid biosynthetic process positive regulation of developmental growth regulation of insulin secretion regulation of steroid biosynthetic process intestinal absorption leukocyte tethering or rolling regulation of nitric-oxide synthase activity negative regulation of transport elastin metabolic process regulation of cell cycle positive regulation of protein kinase B signaling regulation of lipoprotein lipid oxidation adipose tissue development negative regulation of cartilage development negative regulation of glucagon secretion cellular response to L-ascorbic acid cellular response to retinoic acid regulation of brown fat cell differentiation energy homeostasis receptor signaling pathway via STAT bone growth positive regulation of cold-induced thermogenesis regulation of cytokine production involved in inflammatory response regulation of protein localization to nucleus positive regulation of p38MAPK cascade positive regulation of fat cell apoptotic process activation of protein kinase C activity positive regulation of reactive oxygen species metabolic process negative regulation of glutamine transport positive regulation of hepatic stellate cell activation
          It has to be done as per old AB suggested Products section.

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