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Invitrogen
CD45 Monoclonal Antibody (HI30), Pacific Green™
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FIGURE: 1 / 1
CD45 Antibody (C11208) in Flow
Samples were acquired and analyzed using 405 nm excitation and 522/31 nm band pass emission filters on an Attune® Acoustic Focusing Cytometer (Life Technologies, Carlsbad, CA). The histogram shows cells stained with CD45 Mouse Anti-Human Pacific Green™ Conjugate (black line), and unstained cells (gray line).
Product Details
C11208
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
HI30
Immunogen
CD45 antigen
Conjugate
Excitation/Emission Max
415/502 nm
View spectra
Form
Liquid
Storage conditions
4°C
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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