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Invitrogen
Laminin Monoclonal Antibody (A5)
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FIGURE: 1 / 4
Laminin Antibody (MA1-06100) in ICC/IF
Immunofluorescence analysis of laminin was performed using 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Laminin Monoclonal Antibody (Product # MA1-06100) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rat IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dil... View More
Product Details
MA1-06100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig
Published species
Human,
Mouse,
Pig,
Rat,
Rhesus monkey
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
A5
Immunogen
Laminin preparation from the EHS mouse tumor.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-06100 detects laminin in human and mouse samples.
MA1-06100 has sucessfully been used in immunocytochemistry and immunohistochemistry procedures.
The MA1-06100 immunogen is a laminin preparation from the EHS mouse tumor.
Target Information
Laminin is a crucial glycoprotein that plays a significant role in the extracellular matrix, particularly within the basement membrane. It is composed of three subunits: alpha, beta, and gamma chains. These chains are disulfide-linked polypeptides that together form a heterotrimer, resembling the shape of a cross. The human genome encodes 11 genetically distinct laminin chains, which combine to form over 14 different laminin isoforms, each with specific tissue distributions and functions. Laminins can bind to other laminin molecules, as well as integrins and other matrix proteins, providing structural support and influencing cell differentiation, migration, and adhesion. These glycoproteins are vital during early embryonic development, where they contribute to the formation of basement membrane-like structures, even in the absence of type IV collagen. The evolutionary origin of laminin gene families can be traced back to early branching animals, providing insights into metazoan gene family development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: basement membrane protein; dystrophia muscularis; LAMB1; basement membrane protein; Laminin A chain; laminin B1; Laminin B1 chain; laminin B1 subunit 1; Laminin B2 chain; laminin B2 precursor; laminin beta-1 chain; Laminin M chain; laminin S; Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit gamma-1; laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2; laminin, gamma 1 (formerly LAMB2); Laminin-1 subunit alpha; Laminin-1 subunit beta; Laminin-1 subunit gamma; Laminin-10 subunit beta; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-12 subunit alpha; Laminin-12 subunit beta; Laminin-2 subunit alpha; Laminin-2 subunit beta; Laminin-2 subunit gamma; Laminin-3 subunit alpha; Laminin-3 subunit gamma; Laminin-4 subunit alpha; Laminin-4 subunit gamma; Laminin-6 subunit beta; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit beta; Laminin-8 subunit gamma; Laminin-9 subunit gamma; Merosin heavy chain; mutant laminin subunit alpha 2; S-LAM alpha; S-LAM gamma; S-laminin subunit alpha; S-laminin subunit gamma; unnamed protein product
Gene Aliases: 5830440B04; AA408497; C77966; C80098; C81607; CLM; D130003D08Rik; dy; LAMA; Lama-1; LAMA1; LAMA2; Lamb-1; LAMB1; Lamb1-1; LAMB2; LAMC1; LAMM; LIS5; MDC1A; mer; merosin; mKIAA4087; PTBHS; S-LAM-alpha
morphogenesis of an epithelial sheet
cell adhesion
cell surface receptor signaling pathway
tissue development
regulation of cell adhesion
regulation of cell migration
neuron projection development
establishment of epithelial cell apical/basal polarity
positive regulation of cell adhesion
regulation of embryonic development
positive regulation of muscle cell differentiation
retina development in camera-type eye
epithelial tube branching involved in lung morphogenesis
branching involved in salivary gland morphogenesis
retinal blood vessel morphogenesis
regulation of basement membrane organization
positive regulation of integrin-mediated signaling pathway
axon guidance
muscle organ development
Schwann cell differentiation
positive regulation of synaptic transmission, cholinergic
maintenance of blood-brain barrier
negative regulation of cell adhesion
embryo implantation
anatomical structure morphogenesis
cell migration
neuronal-glial interaction involved in cerebral cortex radial glia guided migration
positive regulation of cell migration
substrate adhesion-dependent cell spreading
endodermal cell differentiation
odontogenesis
positive regulation of epithelial cell proliferation
endoderm development
extracellular matrix disassembly
hemidesmosome assembly
protein-containing complex assembly
learning or memory
protein complex assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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