Search
Invitrogen
Lamin A Monoclonal Antibody (133A2)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 11
Lamin A Antibody (MA1-06101) in ICC/IF
Immunofluorescence analysis of Lamin A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Lamin A Monoclonal Antibody (133A2) (Product # MA1-06101) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a d... View More
Product Details
MA1-06101
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Human,
Mouse,
Rat
Published species
Dog,
Human
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
133A2
Immunogen
Partially purified recombinant human lamin A.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Ion-exchange chromatography
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-06101 detects lamin A in human, mouse, rat, bovine and canine samples.
MA1-06101 has sucessfully been used in immunocytochemistry, immunohistochemistry (paraffin), flow cytometry, and Western blotting procedures.
The MA1-06101 immunogen is partially purified recombinant human lamin A.
Target Information
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 70 kDa lamin; epididymis secretory sperm binding protein; lamin A; lamin A protein; lamin A/C; lamin A/C-like 1; lamin C; lamin C protein; lamin C2; lamin-A/C; mandibuloacral dysplasia type A; OTTHUMP00000015843; OTTHUMP00000015846; OTTHUMP00000015847; Prelamin-A/C; progerin; renal carcinoma antigen NY-REN-32; unnamed protein product
Gene Aliases: CDCD1; CDDC; CMD1A; CMT2B1; Dhe; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; MADA; PRO1
UniProt ID: (Human) P02545, (Mouse) P48678
Entrez Gene ID: (Human) 4000, (Bovine) 404144, (Dog) 480124, (Mouse) 16905, (Rat) 60374
nucleus organization
nuclear envelope organization
muscle organ development
establishment of cell polarity
regulation of cell migration
establishment or maintenance of microtubule cytoskeleton polarity
protein localization to nucleus
sterol regulatory element binding protein import into nucleus
ventricular cardiac muscle cell development
negative regulation of release of cytochrome c from mitochondria
positive regulation of cell aging
regulation of protein localization to nucleus
negative regulation of extrinsic apoptotic signaling pathway
protein import into nucleus
nuclear migration
protein localization
negative regulation of cell proliferation
positive regulation of gene expression
heterochromatin assembly
regulation of protein stability
regulation of telomere maintenance
nuclear pore localization
cellular response to hypoxia
negative regulation of mesenchymal cell proliferation
cellular senescence
protein localization to nuclear envelope
negative regulation of cardiac muscle hypertrophy in response to stress
DNA double-strand break attachment to nuclear envelope
spermatogenesis
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support