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Invitrogen
E-selectin Monoclonal Antibody (UZ6)
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FIGURE: 1 / 3
E-selectin Antibody (MA1-06506) in ICC/IF
Immunofluorescence analysis of E-selectin was performed using 70% confluent log phase HUVEC cells treated with 100 U/mL TNF-alpha for 3 Hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with E-selectin Monoclonal Antibody (UZ6) (Product # MA1-06506) at 1:1... View More
Product Details
MA1-06506
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
UZ6
Immunogen
LPS-activated mlEND1 cells expressing E-selectin at their cell surface.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-06506 detects E-selectin in mouse samples.
MA1-06506 has sucessfully been used in Western blotting, FACS, ICC, IF, immunoprecipitation, immunohistochemistry and ELISA.
The MA1-06506 immunogen is LPS-activated mlEND1 cells expressing E-selectin at their cell surface.
Target Information
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD62 antigen-like family member E; CD62E; e selectin; E-selectin; ELAM-1; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2; unnamed protein product
Gene Aliases: CD62E; E-selectin; ELAM; Elam-1; ELAM1; ESEL; LECAM2; SELE; selectin-e
UniProt ID: (Human) P16581, (Mouse) Q00690
Entrez Gene ID: (Human) 6401, (Mouse) 20339
positive regulation of receptor internalization
leukocyte migration involved in inflammatory response
positive regulation of leukocyte migration
inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
phospholipase C-activating G-protein coupled receptor signaling pathway
calcium-mediated signaling
actin filament-based process
response to lipopolysaccharide
response to cytokine
response to tumor necrosis factor
regulation of inflammatory response
leukocyte migration
leukocyte tethering or rolling
response to interleukin-1
cell-cell adhesion via plasma-membrane adhesion molecules
positive regulation of leukocyte tethering or rolling
signal transduction
activation of phospholipase C activity
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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