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Invitrogen
Dynein Monoclonal Antibody (74.1)
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FIGURE: 1 / 8
Dynein Antibody (MA1-070) in ICC/IF
Immunofluorescent analysis of Dynein in U87-MG Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dynein monoclonal antibody (Product # MA1-070) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Dynein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Product Details
MA1-070
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Many,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
74.1
Immunogen
Purified bovine brain cytoplasmic dynein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-070 detects two isoforms of the ~74 kDa polypetide of cytoplasmic dynein.
MA1-070 has been successfully used in Western blot immunofluorescence, and immunoprecipitation procedures. In Western blot analysis of HeLa cell lysate this antibody detects a ~74 kDa protein representing cytoplasmic dynein.
The MA1-070 immunogen is purified bovine brain cytoplasmic dynein.
Target Information
The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains, and intermediate chains of ~74 kDa and ~53-59 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 8 kDa dynein light chain; 8 kDa dynein light chain b; 8kD LC; 8kDa LC; cytoplasmic dynein light polypeptide; cytosolic DH IC-1; DLC8; DLC8b; DNCL1; dynein LC8; Dynein light chain 1, cytoplasmic; Dynein light chain 2, cytoplasmic; Dynein light chain LC8-type 1; Dynein light chain LC8-type 2; dynein light chain-2; dynein, cytoplasmic, light chain 1; dynein, cytoplasmic, light peptide; dynein, cytoplasmic, light polypeptide 1; mPIN; PIN; Protein inhibitor of neuronal nitric oxide synthase; protein inhibitor of nitric oxide synthase; radial spoke 22 homolog; unnamed protein product
Gene Aliases: 1700064A15Rik; 6720463E02Rik; 8kDLC; C87222; CDLC2; DLC1; DLC2; DLC8; DLC8b; DNCL1; DNCL1B; DNCLC1; DYNLL1; DYNLL2; HDLC1; LC8; LC8a; PIN; RSPH22
UniProt ID: (Human) P63167, (Bovine) P61285, (Human) Q96FJ2, (Bovine) Q3MHR3, (Rat) P63170, (Mouse) P63168, (Rat) Q78P75, (Mouse) Q9D0M5
Entrez Gene ID: (Human) 8655, (Bovine) 404151, (Human) 140735, (Bovine) 540369, (Rat) 58945, (Mouse) 56455, (Rat) 140734, (Mouse) 68097
enzyme inhibitor activity
protein binding
enzyme binding
nitric-oxide synthase regulator activity
nitric-oxide synthase inhibitor activity
identical protein binding
macromolecular complex binding
dynein intermediate chain binding
deoxyribonuclease inhibitor activity
scaffold protein binding
motor activity
protein C-terminus binding
protein domain specific binding
protein homodimerization activity
cytoskeletal protein binding
microtubule or microtubule-binding cytoskeletal protein
apoptotic process
cellular response to DNA damage stimulus
microtubule-based process
spermatid development
substantia nigra development
positive regulation of intracellular transport
intraciliary retrograde transport
positive regulation of insulin secretion involved in cellular response to glucose stimulus
negative regulation of phosphorylation
motile cilium assembly
negative regulation of nitric oxide biosynthetic process
DNA strand resection involved in replication fork processing
negative regulation of DNA strand resection involved in replication fork processing
viral process
transcription, DNA-templated
regulation of transcription, DNA-templated
nitric oxide biosynthetic process
transport
neurotransmitter metabolic process
negative regulation of catalytic activity
regulation of catalytic activity
microtubule-based movement
cilium morphogenesis
synaptic target recognition
metabolic process
intracellular transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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