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Invitrogen
SOD2 Monoclonal Antibody (1H6)
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FIGURE: 1 / 15
SOD2 Antibody (MA1-106) in ICC/IF
Immunofluorescence analysis of SOD2 was performed using 70% confluent log phase A549 cells treated with TNF alpha (20 ng/mL for 72h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOD2 Polyclonal Antibody (Product # MA1-106) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluo... View More
Product Details
MA1-106
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
1H6
Immunogen
Full length recombinant protein of human SOD2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-106 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunohistochemistry and immunoprecipitation applications with human samples. This antibody is not suitable for mouse liver tissue in IHC applications and is not suitable for PC12 cells in IF applications.
The MA1-106 immunogen is full length recombinant protein of human SOD2.
Target Information
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu, Zn-SOD (EC-SOD/SOD-3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; gastric cancer-associated lncRNA 1; indophenoloxidase B; IPO B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn superoxide dismutase; Mn-superoxide dismutase; superoxide dismutase; superoxide dismutase 2, mitochondrial; Superoxide dismutase [Mn], mitochondrial; unnamed protein product
Gene Aliases: GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1; Mn-SOD; MNSOD; MVCD6; SOD2
UniProt ID: (Human) P04179
Entrez Gene ID: (Human) 6648
response to reactive oxygen species
response to superoxide
response to hypoxia
release of cytochrome c from mitochondria
liver development
detection of oxygen
vasodilation by acetylcholine involved in regulation of systemic arterial blood pressure
regulation of transcription from RNA polymerase II promoter
glutathione metabolic process
superoxide metabolic process
response to oxidative stress
mitochondrion organization
heart development
locomotory behavior
regulation of blood pressure
negative regulation of cell proliferation
intrinsic apoptotic signaling pathway in response to DNA damage
intrinsic apoptotic signaling pathway in response to oxidative stress
apoptotic mitochondrial changes
response to xenobiotic stimulus
post-embryonic development
response to manganese ion
response to zinc ion
response to selenium ion
response to gamma radiation
positive regulation of hydrogen peroxide biosynthetic process
response to activity
removal of superoxide radicals
respiratory electron transport chain
hemopoiesis
positive regulation of cell migration
response to nutrient levels
oxygen homeostasis
response to lipopolysaccharide
response to L-ascorbic acid
response to silicon dioxide
cellular response to oxidative stress
response to isolation stress
response to immobilization stress
vasodilation
response to hydrogen peroxide
superoxide anion generation
hydrogen peroxide metabolic process
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
positive regulation of nitric oxide biosynthetic process
negative regulation of fat cell differentiation
response to cadmium ion
negative regulation of fibroblast proliferation
neuron development
response to axon injury
erythrophore differentiation
hydrogen peroxide biosynthetic process
protein homotetramerization
response to electrical stimulus
regulation of mitochondrial membrane potential
response to hyperoxia
multicellular organismal iron ion homeostasis
response to magnetism
cellular response to ethanol
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
negative regulation of membrane hyperpolarization
negative regulation of vascular smooth muscle cell proliferation
positive regulation of vascular associated smooth muscle cell apoptotic process
positive regulation of vascular associated smooth muscle cell differentiation involved in phenotypic switching
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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