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Invitrogen
RPE65 Monoclonal Antibody (401.8B11.3D9)
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FIGURE: 1 / 8
RPE65 Antibody (MA1-16578) in ICC/IF
Immunocytochemistry analysis of RPE65 in cultured ARPE19 cells. Samples were incubated with RPE65 monoclonal antibody (Product # MA1-16578) using a dilution of 1:100 in PBS, overnight 4°C. 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T.
Product Details
MA1-16578
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Human,
Mouse,
Non-human primate,
Pig,
Rat,
Xenopus
Published species
Dog,
Human,
Mouse,
Rabbit,
Rat,
Xenopus
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
401.8B11.3D9
Immunogen
Bovine RPE65 microsomal membrane proteins.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
RPE65 is a major protein of the retinal pigment epithelium (RPE). RPE65 is essential for the regeneration of rhodopsin in the visual cycle. Mutations in RPE65 are responsible for certain forms of autosomal recessive severe retinal dystrophy, including Leber Congenital Amaurosis (LCA). Loss of function results in a block in retinoid processing required for the synthesis of 11-cis retinal and the accumulation of retinyl esters in the RPE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: All-trans-retinyl-palmitate hydrolase; BCO family, member 3; Lutein isomerase; Meso-zeaxanthin isomerase; modifier of retinal degeneration 1; RBP-binding membrane protein; retinal pigment epithelium 65-like; retinal pigment epithelium abundant protein RPE65; retinal pigment epithelium specific protein 65; retinal pigment epithelium, 65 kDa; Retinal pigment epithelium-specific 65 kDa protein; retinal pigment epithelium-specific 65kD protein; retinal pigment epithelium-specific protein (65kD); retinal pigment epithelium-specific protein 65kDa; Retinitis pigmentos; retinitis pigmentosa 20 (autosomal recessive); Retinoid isomerohydrolase; Retinol isomerase; RPE65; rpe65 gene for retinal pigment epithelium-specific protein; RPE65, retinoid isomerohydrolase; truncated RPE65; unnamed protein product
Gene Aliases: 65kDa; A930029L06Rik; BCO3; LCA2; Mord1; mRPE65; p63; rd12; RP20; RPE65; sRPE65
UniProt ID: (Chicken) Q9YGX2, (Bovine) Q28175, (Dog) Q9TVB8, (Human) Q16518, (Mouse) Q91ZQ5, (Rat) O70276
Entrez Gene ID: (Chicken) 395700, (Pig) 100516743, (Bovine) 282043, (Dog) 403803, (Human) 6121, (Mouse) 19892, (Rat) 89826
phosphatidylserine binding
retinal isomerase activity
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
isomerase activity
phosphatidylcholine binding
metal ion binding
retinol isomerase activity
all-trans-retinyl-palmitate hydrolase, 11-cis retinol forming activity
all-trans-retinyl-ester hydrolase, 11-cis retinol forming activity
cardiolipin binding
identical protein binding
beta-carotene 15,15'-monooxygenase activity
protein binding
hydrolase activity
oxygenase
retinoid metabolic process
visual perception
retinol metabolic process
response to stimulus
oxidation-reduction process
zeaxanthin biosynthetic process
retina homeostasis
neural retina development
lipid metabolic process
vitamin A metabolic process
circadian rhythm
response to light stimulus
retinal metabolic process
camera-type eye development
detection of light stimulus involved in visual perception
regulation of rhodopsin gene expression
insulin receptor signaling pathway
retina development in camera-type eye
retina morphogenesis in camera-type eye
cellular response to electrical stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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