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          • Primary Antibodies ›
          • p300 Antibodies

          Invitrogen

          p300 Monoclonal Antibody (RW128)

          Advanced Verification
          1 Published Figure
          5 References
          View all (22) p300 antibodies

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          Cite p300 Monoclonal Antibody (RW128)

          Additional Information:
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          • Antibody Testing Data (8)
          • Published Figures (1)
          • Advanced Verification (2)
          p300 Antibody in Immunocytochemistry (ICC/IF)
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          p300 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

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          p300 Antibody (MA1-16608) in ICC/IF

          Immunofluorescence analysis of p300 Monoclonal Antibody (RW128) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with p300 Monoclonal Antibody (RW128) (Product # MA1-16608) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Ale... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          p300 Antibody in Immunocytochemistry (ICC/IF)
          p300 Antibody in Immunocytochemistry (ICC/IF)
          p300 Antibody in Immunocytochemistry (ICC/IF)
          p300 Antibody in Immunocytochemistry (ICC/IF)
          p300 Antibody in Western Blot (WB)
          p300 Antibody in Western Blot (WB)
          p300 Antibody in Flow Cytometry (Flow)
          p300 Antibody in Flow Cytometry (Flow)
          p300 Antibody in Western Blot (WB)
          p300 Antibody
          p300 Antibody
          p300 Monoclonal Antibody (RW128)

          Product Details

          MA1-16608

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:250-1:500
          View 4 publications 4 publications

          Immunocytochemistry (ICC/IF)

          2-5 µg/mL
          -

          Flow Cytometry (Flow)

          1 µg/mL
          -

          Immunoprecipitation (IP)

          1:10-1:500
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mustelid, Mouse, Non-human primate, Rat

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          RW128

          Immunogen

          Fusion protein containing residues 1572-2371 of human KAT3B/p300.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_568667

          Product Specific Information

          Suggested positive control: HeLa nuclear extract.

          Target Information

          EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: E1A binding protein p300; E1A-associated protein p300; E1A-binding protein, 300kD; Histone acetyltransferase p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; p300 HAT; Protein 2-hydroxyisobutyryltransferase p300; Protein isonicotinyltransferase p300; Protein lactyltransferas p300; Protein propionyltransferase p300

          View more View less

          Gene Aliases: A430090G16; A730011L11; EP300; KAT3B; MKHK2; P300; p300 HAT; RSTS2

          View more View less

          UniProt ID: (Human) Q09472, (Mouse) B2RWS6

          View more View less

          Entrez Gene ID: (Human) 2033, (Mouse) 328572, (Rat) 170915

          View more View less

          Function(s)
          transcription cofactor binding transcription coactivator binding p53 binding DNA binding chromatin binding damaged DNA binding transcription cofactor activity transcription coactivator activity histone acetyltransferase activity lysine N-acetyltransferase activity, acting on acetyl phosphate as donor protein binding beta-catenin binding zinc ion binding H3 histone acetyltransferase activity H4 histone acetyltransferase activity acetyltransferase activity transferase activity transferase activity, transferring acyl groups ligand-dependent nuclear receptor binding chromatin DNA binding histone acetyltransferase activity (H3-K18 specific) histone H2B acetyltransferase activity histone acetyltransferase activity (H3-K27 specific) metal ion binding tau protein binding androgen receptor binding NF-kappaB binding RNA polymerase II sequence-specific DNA binding transcription factor binding peptide-lysine-N-acetyltransferase activity protein propionyltransferase activity pre-mRNA intronic binding STAT family protein binding peptide 2-hydroxyisobutyryltransferase activity peptide lactyltransferase (CoA-dependent) activity histone lactyltransferase (CoA-dependent) activity acetylation-dependent protein binding peptide crotonyltransferase activity peptide butyryltransferase activity histone crotonyltransferase activity histone butyryltransferase activity DNA-binding transcription factor binding histone reader activity histone H3K122 acetyltransferase activity RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding core promoter proximal region DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding antigen binding protein C-terminus binding transcription factor binding protein kinase binding protein complex binding activating transcription factor binding nuclear hormone receptor binding glucocorticoid receptor binding peroxisome proliferator activated receptor binding bHLH transcription factor binding SMAD binding mitogen-activated protein kinase binding protein antigen binding histone modifying enzyme
          Process(es)
          autophagosome assembly negative regulation of transcription from RNA polymerase II promoter response to hypoxia somitogenesis thigmotaxis behavioral defense response stimulatory C-type lectin receptor signaling pathway gluconeogenesis glycolytic process regulation of glycolytic process chromatin remodeling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein acetylation internal protein amino acid acetylation apoptotic process I-kappaB kinase/NF-kappaB signaling JAK-STAT cascade nervous system development heart development skeletal muscle tissue development learning or memory circadian rhythm lipid biosynthetic process organ morphogenesis regulation of autophagy negative regulation of autophagy positive regulation of gene expression regulation of mitochondrion organization positive regulation of neuron projection development N-terminal peptidyl-lysine acetylation internal peptidyl-lysine acetylation B cell differentiation platelet formation lung development positive regulation of transforming growth factor beta receptor signaling pathway negative regulation of protein complex assembly protein destabilization cellular response to nutrient levels negative regulation of protein oligomerization cellular response to UV multicellular organism growth megakaryocyte development endodermal cell differentiation swimming TORC1 signaling TORC2 signaling positive regulation of protein import into nucleus intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator protein kinase B signaling response to estrogen positive regulation by host of viral transcription fat cell differentiation negative regulation of gluconeogenesis positive regulation of gene expression, epigenetic positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade rhythmic process protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of protein kinase B signaling canonical Wnt signaling pathway face morphogenesis regulation of androgen receptor signaling pathway peptidyl-lysine propionylation protein localization to chromatin cellular response to leucine tricarboxylic acid metabolic process T-helper 17 cell lineage commitment regulation of tubulin deacetylation peptidyl-lysine crotonylation peptidyl-lysine butyrylation regulation of cellular response to heat positive regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of T-helper 17 cell lineage commitment positive regulation of protein phosphorylation transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response cell cycle response to glucose positive regulation of glycoprotein biosynthetic process positive regulation of cell death positive regulation of muscle atrophy histone acetylation cell differentiation positive regulation of cell growth negative regulation of cellular metabolic process positive regulation of cellular metabolic process positive regulation of protein binding positive regulation of collagen biosynthetic process positive regulation of protein import into nucleus, translocation positive regulation of histone acetylation response to drug negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of DNA binding histone H3 acetylation histone H4 acetylation histone H2B acetylation positive regulation of translation positive regulation of axon extension positive regulation of cell size positive regulation of proteolysis positive regulation of protein secretion cartilage development response to calcium ion regulation of angiotensin metabolic process positive regulation of sarcomere organization negative regulation of cell death protein-DNA complex assembly response to dexamethasone positive regulation of protein acetylation regulation of nucleic acid-templated transcription negative regulation of miRNA metabolic process liver development memory response to organic cyclic compound response to cobalt ion response to retinoic acid response to tumor necrosis factor cellular response to drug cellular response to trichostatin A response to hydrogen peroxide response to ethanol digestive tract development cellular response to hydrogen peroxide response to fatty acid cellular response to antibiotic cellular response to retinoic acid cellular response to cAMP cellular response to glucose stimulus cellular response to mineralocorticoid stimulus cellular response to organic cyclic compound cellular response to dexamethasone stimulus cellular response to nerve growth factor stimulus
          It has to be done as per old AB suggested Products section.

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