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Invitrogen
alpha/beta Tubulin Monoclonal Antibody (TU-10)
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FIGURE: 1 / 4
alpha/beta Tubulin Antibody (MA1-19400) in ICC/IF
Immunofluorescence analysis of alpha/beta Tubulin was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha/beta Tubulin (TU-10) Mouse Monoclonal Antibody (Product # MA1-19400) at 2µg/mL in 0.1% BSA and in... View More
Product Details
MA1-19400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Many,
Mouse,
Pig,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
TU-10
Immunogen
Microtubule proteins from porcine brain.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
TBS, pH 8
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes alpha, beta-tubulin heterodimer, a basic intracellular structural unit of microtubules. Alpha- and beta-tubulins form approximately 100 kDa tubulin heterodimer, a globular protein that polymerizes to form microtubules.
Western Blot: Reducing conditions.
Target Information
Tubulin protein is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. There are at least six isotypes of both alpha- and beta- tubulin in human cells, which are distinguished by slightly different amino acid sequences and encoded by a large, multigene family that has been highly conserved throughout evolution. Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Anti-microtubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Alpha-tubulin 1; Alpha-tubulin 3; Alpha-tubulin isotype M-alpha-1; beta 5-tubulin; beta Ib tubulin; epididymis secretory sperm binding protein; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; Tubulin alpha; tubulin alpha 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-3 chain; Tubulin B-alpha-1; tubulin beta; Tubulin beta chain; Tubulin beta-1 chain; Tubulin beta-5 chain; tubulin, alpha 1; tubulin, alpha, brain-specific; tubulin, beta 1; tubulin, beta 1 class VI; tubulin, beta 2A class IIa; tubulin, beta family 1; tubulin, beta polypeptide; unnamed protein product; XTP3TPATP1; α/β-tubulin
Gene Aliases: 2810484G07Rik; B-ALPHA-1; CDCBM6; CSCSC1; LIS3; M(beta)1; M40; MACTHC1; OK/SW-cl.56; Tuba-1; Tuba1; TUBA1A; TUBA3; TUBB; TUBB1; TUBB2A; TUBB5
UniProt ID: (Human) Q71U36, (Human) P07437, (Pig) Q767L7, (Human) Q9H4B7, (Mouse) P68369, (Rat) P05210, (Mouse) A2AQ07
Entrez Gene ID: (Pig) 100135051, (Human) 7846, (Human) 203068, (Pig) 733686, (Pig) 100134983, (Human) 81027, (Mouse) 22142, (Rat) 64158, (Mouse) 545486, (Rat) 679312
GTPase activity
nucleotide binding
structural molecule activity
structural constituent of cytoskeleton
protein binding
GTP binding
hydrolase activity
identical protein binding
macromolecular complex binding
metal ion binding
protein heterodimerization activity
protein domain specific binding
ubiquitin protein ligase binding
GTPase activating protein binding
MHC class I protein binding
tubulin
microtubule cytoskeleton organization
mitotic cell cycle
neuron migration
startle response
intracellular protein transport
microtubule-based process
centrosome cycle
smoothened signaling pathway
memory
locomotory behavior
adult locomotory behavior
visual learning
response to mechanical stimulus
glial cell differentiation
gene expression
dentate gyrus development
cerebellar cortex morphogenesis
hippocampus development
pyramidal neuron differentiation
cerebral cortex development
neurogenesis
neuron differentiation
sperm motility
adult behavior
cytoskeleton-dependent intracellular transport
response to tumor necrosis factor
locomotory exploration behavior
microtubule polymerization
forebrain morphogenesis
homeostasis of number of cells within a tissue
regulation of synapse organization
synapse organization
cell division
neuron apoptotic process
motor behavior
cellular response to calcium ion
organelle transport along microtubule
neuron projection arborization
response to L-glutamate
natural killer cell mediated cytotoxicity
spindle assembly
odontoblast differentiation
platelet formation
thyroid gland development
thyroid hormone transport
platelet aggregation
protein polymerization
metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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