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Invitrogen
SSEA1 Monoclonal Antibody (MEM-158), FITC
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FIGURE: 1 / 2
SSEA1 Antibody (MA1-19562) in Flow
Separation of human neutrophil granulocytes (red-filled) from CD15 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD15 (MEM-158) FITC Monoclonal antibody (Product # MA1-19562) using a dilution of 20 µL reagent/100 µL of peripheral whole blood.
Product Details
MA1-19562
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgM, kappa
Class
Monoclonal
Type
Antibody
Clone
MEM-158
Immunogen
Human granulocytes.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
TBS, pH 8.0, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on the surface of granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.
This antibody will not cross-react with porcine, bovine or sheep.
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid-specific; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1
Gene Aliases: CD15; ELFT; FCT3A; FUC-TIV; FUT4; FUTIV; LeX; SSEA-1
UniProt ID: (Human) P22083
Entrez Gene ID: (Human) 2526
carbohydrate metabolic process
protein glycosylation
protein N-linked glycosylation
protein O-linked glycosylation
sphingolipid metabolic process
glycosphingolipid biosynthetic process
inflammatory response
oligosaccharide metabolic process
oligosaccharide biosynthetic process
fucosylation
L-fucose catabolic process
lymphocyte migration into lymph node
Lewis x epitope biosynthetic process
positive regulation of neutrophil migration
regulation of leukocyte cell-cell adhesion
positive regulation of leukocyte tethering or rolling
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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