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Invitrogen
THRA/THRB Monoclonal Antibody (C3)
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FIGURE: 1 / 24
THRA/THRB Antibody (MA1-215) in ICC/IF
Immunofluorescent analysis of Thyroid Hormone Receptor in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Thyroid Hormone Receptor monoclonal antibody (Product # MA1-215) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Thyroid ... View More
Product Details
MA1-215
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Hamster,
Human,
Mouse,
Non-human primate,
Rat,
Xenopus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
C3
Immunogen
Purified fragment of human TR beta-1 corresponding to residues 201-456.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-215 detects thyroid hormone receptor (TR) from human tissues. This antibody recognizes TR beta-1 and also cross-reacts with TR alpha-1.
MA1-215 has been successfully used in Western blot, IF, immunoprecipitation, and gel shift procedures. By Western blot, this antibody detects a 55 and a 52 kDa protein representing recombinant human TR beta-1 expressed in E. coli. MA1-215 immunoprecipitates 52 kDa and 55 kDa proteins, representing TR beta-1 and a 48 kDa protein representing TR alpha-1 expressed in E. coli.
The MA1-215 immunogen is a purified fragment of human TR beta-1 corresponding to amino acid residues 201-456. This antibody recognizes an epitope in the hormone binding domain of hTR beta-1, amino acid residues 235-414. This sequence is conserved in hTR alpha-1.
Target Information
The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: c-erbA protooncogene; c-erbA-1; c-erbA-2; c-erbA-alpha; c-erbA-beta; EAR-7; ERBA-related 7; nuclear receptor; Nuclear receptor subfamily 1 group A member 1; Nuclear receptor subfamily 1 group A member 2; oncogene ERBA2; THR; THRA; Thyroid hormone receptor alpha; thyroid hormone receptor alpha 1; Thyroid hormone receptor beta; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian); thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); TR; triiodothyronine receptor; unnamed protein product; V-erbA-related protein 7
Gene Aliases: AR7; c-erbA; c-ERBA-1; C-ERBA-2; C-ERBA-BETA; CHNG6; EAR7; ERB-T-1; ERBA; ERBA1; ERBA2; GRTH; NR1A1; NR1A2; PRTH; THR1; THRA; THRA1; THRA2; THRalpha; THRalpha1; THRalpha2; THRB; THRB1; THRB2; THRbeta; THRbeta1; Thrbeta2; TRalpha; TRalpha1; TRalpha2; TRb; TRbeta; TRbeta1
UniProt ID: (Human) P10827, (Human) P10828
Entrez Gene ID: (Human) 7067, (Human) 7068
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
TBP-class protein binding
protein domain specific binding
chromatin DNA binding
sequence-specific DNA binding
macromolecular complex binding
metal ion binding
thyroid hormone binding
general transcription initiation factor binding
transcription coactivator binding
chromatin binding
enzyme binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
thyroid hormone mediated signaling pathway
positive regulation of thyroid hormone receptor signaling pathway
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
sensory perception of sound
negative regulation of female receptivity
regulation of heart contraction
female courtship behavior
organ morphogenesis
cell differentiation
mRNA transcription from RNA polymerase II promoter
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
retinal cone cell development
retinoic acid receptor signaling pathway
Type I pneumocyte differentiation
cellular response to thyroid hormone stimulus
retinal cone cell apoptotic process
cartilage condensation
ossification
regulation of thyroid hormone mediated signaling pathway
transcription from RNA polymerase II promoter
learning or memory
response to cold
hormone-mediated signaling pathway
negative regulation of RNA polymerase II transcriptional preinitiation complex assembly
erythrocyte differentiation
thyroid gland development
regulation of myeloid cell apoptotic process
positive regulation of female receptivity
regulation of lipid catabolic process
positive regulation of cold-induced thermogenesis
negative regulation of DNA-templated transcription, initiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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