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Invitrogen
HP1 alpha Monoclonal Antibody
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FIGURE: 1 / 4
HP1 alpha Antibody (MA1-218) in ICC/IF
Immunofluorescent analysis of CBX5 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a CBX5 mouse monoclonal antibody (Product # MA1-218) at a concentration of 2.5 µg/mL in blocking buffer for 1 hour at room temperature, and then incubate... View More
Product Details
MA1-218
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Immunogen
Purified recombinant fragment of human CBX5 (AA: 1-191) expressed in E. Coli
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-218 detects CBX5 from human and non-human primate samples. CBX5 has an approximate molecular weight of 22.2kDa.
MA1-218 has been successfully used in Western blot, immunofluorescence,and immunohistochemistry applications.
The MA1-218 Immunogen is the purified recombinant fragment of human CBX5 (AA: 1-191) expressed in E. Coli.
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 25 kda chromosomal autoantigen; Antigen p25; CBX5; Cbx5 antibody; chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox protein homolog 5; epididymis luminal protein 25; Heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HGNC:1555; HP-1 alpha antibody; HP1 alpha; HP1 alpha homolog; HP1-ALPHA; HP1Hs alpha; HP1Hs-alpha; unnamed protein product
Gene Aliases: CBX5; HEL25; HP1; HP1A; HP1alpha
UniProt ID: (Human) P45973
Entrez Gene ID: (Human) 23468
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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