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Invitrogen
FGF2 Monoclonal Antibody (FB-8)
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FIGURE: 1 / 9
FGF2 Antibody (MA1-24682) in ICC/IF
Immunofluorescence analysis of FGF2 was performed using 80% confluent log phase BJ and T-47D, control and untreated, cells. Cells were fixed with 4% paraformaldehyde for 10 mins, permeabilized with 0.1% Triton™ X-100 for 10 mins, and blocked with 2% BSA for 45 mins at room temperature. The cells were labeled with FGF2 Monoclonal Antibody (FB-8) (Product # MA1-24682) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Produ... View More
Product Details
MA1-24682
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
FB-8
Immunogen
Recombinant human FGF2.
Conjugate
Form
Liquid
Concentration
2.3 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: recombinant human FGF2.
This antibody does not cross react with human acidic FGF.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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