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Invitrogen
Cytokeratin 5/6/8/17/10 Monoclonal Antibody (MNF116)
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FIGURE: 1 / 6
Cytokeratin 5/6/8/17/10 Antibody (MA1-26237) in ICC/IF
Immunofluorescence analysis of Cytokeratin Pan was performed using 70% confluent log phase A-431 cells. The cells were fixed and permeabilized with ice-cold acetone at 4°C for 5 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin Pan Monoclonal Antibody (MNF116) (Product # MA1-26237, 1:100 dilution) in 0.1% BSA, incubated at 4 degree celsius overnight and ... View More
Product Details
MA1-26237
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MNF116
Immunogen
Crude extract of splenic cells from a nude mouse engrafted with MCF-7 cells.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: Skin.
This antibody reacts Cytokeratin 5, Cytokeratin 6, Cytokeratin 8, Cytokeratin 17 and Cytokeratin 19.
Target Information
Cytokeratins (CKs) are intermediate filament proteins essential for the structural integrity and function of epithelial cells. They are categorized into various types based on their distribution and expression patterns. CK5 is primarily expressed in basal epithelial cells, particularly in the breast, and serves as a marker for basal-like breast carcinomas. CK6, though functionally distinct from CK5, is often studied concurrently due to its expression in squamous cell types and squamous cell carcinomas. CK8 is a marker for luminal epithelial cells and is predominantly expressed in various carcinomas, including hepatocellular carcinoma. CK17 is associated with the basal-like phenotype in certain cancers, notably correlating with poor prognosis in breast cancer and the BRCA1 mutation. CK10 is typically expressed in keratinizing squamous epithelium and is pivotal in skin differentiation. Understanding the differential expression of these cytokeratins aids in the histological diagnosis and prognosis of epithelial carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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