Invitrogen

TIMP1 Monoclonal Antibody (F31 P2 A5)

Name: TIMP1 Monoclonal Antibody (F31 P2 A5)
Brand: Invitrogen
SKU: MA1-773

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3 Published Figures

12 References

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Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Additional Information:

TIMP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

TIMP1 Antibody (MA1-773) in ICC/IF

Immunofluorescence analysis of TIMP1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TIMP1 (F31 P2 A5) Mouse Monoclonal Antibody (Product # MA1-773) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)... View More

TIMP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

TIMP1 Antibody — Fig. 5 KDM3A activates the transcription of Timp1 in cardiomyocytes. Cardiomyocytes (CM) and cardiac fibroblasts (cFb) were isolated from WT and Kdm3a-Tg mouse hearts at week 6 post-Sham and TAC surgery. a - e Transcripts of fetal genes and fibrosis-related ECM genes were measured by qRT-PCR, normalized against internal Gapdh, and expressed relative to WT Sham CM. n = 3 +- SEM , * , # p < 0.05 by student t test. *, WT TAC vs WT Sham. # , Tg-TAC vs WT-TAC. f Cardiomyocytes from Kdm3a -Tg mouse hearts at week 6 post-Sham and TAC surgery were isolated and used for ChIP assay with antibodies against KDM3A (left panel) or H3K9me2 (right panel). The relative occupancies of KDM3A and H3K9me2 at Timp1 promoter were normalized against Input and expressed relative to Sham control. n = 3 +- SEM , *p < 0.05 ( t test). g Timp1-Luciferase reporter was transfected in combination with plasmids as indicate into 293 T cells. Cells were harvested 48 h after transfection. Luciferase activities were normalized against co-transfected beta-galactosidase, expressed relative to vector-transfected cells. n = 3 +- SEM. (h-k) Kdm3a- Tg mice were transduced with adenovirus AAV9 expressing control or Timp1 shRNA and subjected to Sham or TAC surgery. Hearts were echoed and harvested 5 weeks post-surgery. h WB of TIMP1 and GAPDH, i HW/BW, j %FS, and k percent of fibrotic area of Sham (S), TAC (T) Kdm3a-Tg mice transduced with control or Timp1 shRNA. n = 7 +- SEM, * p < 0.05 (ANOVA) {KD}

Immunogen image

Immunogen Image TIMP1 Monoclonal Antibody (F31 P2 A5)

Product Details

MA1-773

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 2 publications 2 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rhesus monkey

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

F31 P2 A5

Immunogen

Synthetic peptide corresponding to residues F(72) Q A L G D A A D I R(82) of mouse TIMP-1.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_889482

Product Specific Information

MA1-773 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.

MA1-773 detects TIMP-1 from human samples.

MA1-773 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry procedures. By Western blot, MA1-773 detects a ~30 kDa band representing TIMP-1 from K562 cell lysate. It is recommended to block with 3% BSA.

The MA1-773 immunogen is a synthetic peptide corresponding to residues F(72) Q A L G D A A D I R(82) of mouse TIMP-1.This sequence is 100% conserved in some non-human primate TIMP-1.

Target Information

TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Collagenase inhibitor; Collagenase inhibitor 16C8 fibroblast; EPA; epididymis secretory sperm binding protein; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; Metalloproteinase inhibitor; Metalloproteinase inhibitor 1; MMP inhibitor; prefibroblast collagenase inhibitor; RP1-230G1.3; TIMP; TIMP 1; TIMP-1; TIMP1; tissue inhibitor of matrix metalloproteinase-1; tissue inhibitor of metalloproteinase-1; Tissue inhibitor of metalloproteinases 1; TPA-induced protein; TPA-S1; unnamed protein product

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Gene Aliases: CLGI; EPA; EPO; HCI; TIMP; TIMP-1; TIMP1; TPA-S1

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UniProt ID: (Rhesus monkey) Q95KL9, (Human) P01033, (Mouse) P12032

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Entrez Gene ID: (Rhesus monkey) 574348, (Human) 7076, (Mouse) 21857

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Function(s)

protease binding enzyme inhibitor activity cytokine activity protein binding growth factor activity metalloendopeptidase inhibitor activity zinc ion binding peptidase inhibitor activity metal ion binding protease inhibitor

Process(es)

connective tissue replacement involved in inflammatory response wound healing signal transduction positive regulation of cell proliferation response to hormone negative regulation of endopeptidase activity response to cytokine negative regulation of apoptotic process negative regulation of catalytic activity response to peptide hormone negative regulation of membrane protein ectodomain proteolysis cartilage development cellular response to UV-A negative regulation of trophoblast cell migration cellular response to peptide negative regulation of metallopeptidase activity regulation of integrin-mediated signaling pathway cell activation negative regulation of peptidase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TIMP1 Monoclonal Antibody (F31 P2 A5)

TIMP1 Antibody (MA1-773) in ICC/IF

Product Details

MA1-773

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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