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Invitrogen
TIMP2 Monoclonal Antibody (F27 P3 A4)
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FIGURE: 1 / 9
TIMP2 Antibody (MA1-774) in ICC/IF
Immunofluorescence analysis of TIMP2 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TIMP2 (F27 P3 A4) Mouse Monoclonal Antibody (Product # MA1-774) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) ... View More
Product Details
MA1-774
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Plant
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
F27 P3 A4
Immunogen
Synthetic peptide corresponding to residues D(56) S G N D I Y G N P I(66) of mouse TIMP-2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-774 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.
MA1-774 detects TIMP-2 from mouse samples.
MA1-774 has been successfully used in Western blot, IF and immunohistochemistry procedures. By Western blot, MA1-774 detects a ~18-24 kDa band representing TIMP-2 from mouse platelets. It is recommended to block with 3% BSA.
The MA1-774 immunogen is a synthetic peptide corresponding to residues D(56) S G N D I Y G N P I(66) of mouse TIMP-2. This sequence is 100% conserved in human, rat, rabbit, chicken, and bovine TIMP-2
Target Information
TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CSC-21K; Metalloproteinase inhibitor 2; metalloproteinase inhibitor precursor; metalloproteinase-2 inhibitor precursor; testicular secretory protein Li 57; TIMP 2; TIMP-2; Tissue inhibitor of metalloproteinases 2; unnamed protein product
Gene Aliases: CSC-21K; D11Bwg1104e; DDC8; Timp-2; TIMP2
UniProt ID: (Human) P16035
Entrez Gene ID: (Human) 7077, (Mouse) 21858
response to hormone
response to cytokine
negative regulation of membrane protein ectodomain proteolysis
negative regulation of metallopeptidase activity
negative regulation of cell proliferation
negative regulation of peptidase activity
negative regulation of endopeptidase activity
regulation of cAMP metabolic process
regulation of Rap protein signal transduction
positive regulation of catalytic activity
negative regulation of catalytic activity
regulation of MAPK cascade
positive regulation of MAPK cascade
regulation of neuron differentiation
positive regulation of neuron differentiation
positive regulation of adenylate cyclase activity
negative regulation of proteolysis
negative regulation of mitotic cell cycle
negative regulation of Ras protein signal transduction
cellular response to organic substance
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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