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Invitrogen
alpha Tubulin Monoclonal Antibody (YL1/2)
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FIGURE: 1 / 46
alpha Tubulin Antibody (MA1-80017) in ICC/IF
Immunofluorescence analysis of alpha Tubulin was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Tubulin (YL1/2) Rat Monoclonal Antibody at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temper... View More
Product Details
MA1-80017
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Fruit fly,
Human,
Mouse,
Plant,
Pig,
Rabbit,
Rat,
Xenopus,
Yeast
Published species
Arthropod,
Bacteria,
Cnidarian,
Dog,
Fruit fly,
Fungi,
Human,
Insect,
Monkey,
Mouse,
Non-human primate,
Plant,
Protozoa,
Rabbit,
Rat,
Yeast
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
YL1/2
Immunogen
Yeast tubulin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This clone recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this antibody appears to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).
MA1-80017 is predicted to react with amphibia, birds, and echinoderm based on sequence homology. Does not react with nephrotoma suturalis.
This antibody is suitable for use as a loading control.The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin.
Target Information
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: &agr-tubulin 84B; alpha tubulin; alpha tubulin 84B; alpha-Tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; alpha-Tubulin 84B; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; alpha-tubulin84B; alpha1 tubulin; alpha1-tubulin; alpha[[1]] tubulin; alpha[[1]]-tubulin; alphaT; alphaTub84B-PA; alphatubulin; alphaTubulin 84B; alphaTubulin84B; AT25469p; beta-tubulin T beta15 (aa 1-445); CG1913-PA; class II beta-tubulin isotype; class IIb beta-tubulin; epididymis secretory sperm binding protein; FLJ30169; gangliocyte protein related to virus stimulation; H2 ALPHA; hum-a-tub1; hum-a-tub2; MGC8685; similar to beta tubulin; T beta-15; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; Tubulin alpha; tubulin alpha 3; Tubulin alpha chain-like 3; tubulin alpha-1; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; tubulin alpha-3C/D chain; Tubulin alpha-3D chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; tubulin alpha1; tubulin alpha1 promoter; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin(alpha1); tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 2; tubulin, alpha 4; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, beta 2; tubulin, beta 2B; tubulin, beta polypeptide paralog; Tubulin-alpha1; tubulin1alpha; tubulin84B; unnamed protein product
Gene Aliases: 1t; 2410129E14Rik; a-tub84B; a1t; ALPHA 84C; alpha-Tub; alpha-Tub84B; alpha-tubulin; alpha-tubulin 84B; Alpha-Tubulin84B; alpha1; alpha1t; alpha1tub; alpha1tub84B; alpha84B; alphaT; alphat-1; alphaTUB; alphaTub1; alphaTub84; alphaTUB84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; aTub84B; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; BEST:LD32507; brdp; CDCBM7; CG1913; chr3R:2914276..2914416; clone 1.59; clone 2.30; CMYO26; DM1alpha; Dmel\CG1913; Dmel_CG1913; DTA1; FTDALS9; H2-ALPHA; K-ALPHA-1; l(3)84Bd; l(3)g3; LIS3; M[a]4; M[a]6; ms(3)nc33; nc33; OZEMA23; OZEMA24; PMGYSA; RGD1309427; RGD1565476; SPAX11; T; Talpha1; TBA1_DROME; Tub; Tub 84B; TUB1; Tub1C; Tub84B; Tuba-1; TUBA1; TUBA1A; tuba1a-a; tuba1a-b; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; Tuba84B; tubal2; TUBAL3; Tubalpha1; Tubb2; Tubb2a; TUBB2B; tubulin; XELAEV_18019849mg; XELAEV_18020901mg; YML085C
UniProt ID: (Baker's yeast) P09733, (Human) Q71U36, (Human) P68363, (Pig) Q2XVP4, (Human) Q9BQE3, (Human) P0DPH7, (Human) Q6PEY2, (Human) P68366, (Human) A6NHL2, (Human) Q9BVA1, (Mouse) P68369, (Rat) P05210, (Mouse) P05213, (Rat) Q6P9V9, (Mouse) P68373, (Rat) Q6AYZ1, (Mouse) P68368, (Rat) Q5XIF6, (Mouse) Q3UX10, (Mouse) Q9CWF2, (Rat) Q3KRE8, (Fruit fly) P06603
Entrez Gene ID: (Dog) 106557476, (Baker's yeast) 854889, (Pig) 100135051, (Human) 7846, (Human) 10376, (Pig) 733594, (Rabbit) 100009327, (Human) 84790, (Human) 7278, (Human) 112714, (Pig) 100151951, (Dog) 478918, (Human) 7277, (Rabbit) 100354352, (Human) 79861, (Rabbit) 100353478, (Dog) 487148, (Pig) 100623355, (Human) 347733, (Dog) 478702, (Pig) 100153507, (Mouse) 22142, (Rat) 64158, (Mouse) 22143, (Rat) 500929, (Mouse) 22146, (Rat) 300218, (Mouse) 22145, (Rat) 316531, (Rat) 291287, (Mouse) 238463, (Mouse) 73710, (Rat) 291081, (Fruit fly) 40848, (Xenopus) 431994, (Xenopus) 100036943
nucleotide binding
molecular_function
structural constituent of cytoskeleton
GTP binding
GTPase activity
structural molecule activity
protein binding
hydrolase activity
identical protein binding
macromolecular complex binding
metal ion binding
protein heterodimerization activity
double-stranded RNA binding
ubiquitin protein ligase binding
enzyme binding
protein kinase binding
protein domain specific binding
tubulin
mitotic sister chromatid segregation
microtubule cytoskeleton organization
mitotic cell cycle
nuclear division
microtubule-based process
nuclear migration along microtubule
homologous chromosome segregation
nuclear migration by microtubule mediated pushing forces
neuron migration
startle response
intracellular protein transport
centrosome cycle
smoothened signaling pathway
memory
locomotory behavior
adult locomotory behavior
visual learning
response to mechanical stimulus
glial cell differentiation
gene expression
dentate gyrus development
cerebellar cortex morphogenesis
hippocampus development
pyramidal neuron differentiation
cerebral cortex development
neurogenesis
neuron differentiation
sperm motility
adult behavior
cytoskeleton-dependent intracellular transport
response to tumor necrosis factor
locomotory exploration behavior
microtubule polymerization
forebrain morphogenesis
homeostasis of number of cells within a tissue
regulation of synapse organization
synapse organization
cell division
neuron apoptotic process
motor behavior
cellular response to calcium ion
organelle transport along microtubule
neuron projection arborization
response to L-glutamate
cellular response to interleukin-4
cytoskeleton organization
nervous system development
modulation of synaptic transmission
positive regulation of axon guidance
embryonic brain development
protein polymerization
metabolic process
biological_process
lysosome localization
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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