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Invitrogen
AKAP9 Monoclonal Antibody (17G10)
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FIGURE: 1 / 4
AKAP9 Antibody (MA1-803) in ICC/IF
Immunofluorescent analysis of AKAP450 in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP450 monoclonal antibody (Product # MA1-803) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). AKAP450 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Product Details
MA1-803
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
17G10
Immunogen
Synthetic peptide corresponding to residues Q(31) F R Q R K A Q S D G Q S P S(45) of human AKAP450.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-803 detects the AKAP450 in human and mouse cells.
MA1-803 has been successfully used in Immunoprecipitation, immunohistochemistry, and immunofluorescence procedures.
The MA1-803 immunogen is a synthetic peptide corresponding to residues Q(31) F R Q R K A Q S D G Q S P S(45) of human AKAP450.
Target Information
The A-kinase anchor proteins are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in at least two isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A kinase (PRKA) anchor protein (yotiao) 9; A kinase (PRKA) anchor protein 9; A-kinase anchor protein 350 kDa; A-kinase anchor protein 450 kDa; A-kinase anchor protein 9; A-kinase anchoring protein; putative centrosomally assiciated AKAP; AKAP 120-like protein; AKAP 350; AKAP 450; AKAP-9; AKAP9-BRAF fusion protein; centrosomally-associated AKAP; Centrosome- and Golgi-localized PKN-associated protein; centrosome- and golgi-localized protein kinase N-associated protein; CG-NAP; hypothetical; AKAP450; kinase N-associated protein; PRKA9; Protein hyperion; protein kinase A anchoring protein 9; Protein kinase A-anchoring protein 9; protein phosphatase 1, regulatory subunit 45; Protein yotiao; unnamed protein product
Gene Aliases: 5730481H23Rik; AKAP-9; AKAP350; AKAP450; AKAP9; AW545847; C79026; CG-NAP; G1-448-15; HYPERION; KIAA0803; LQT11; mei2-5; mKIAA0803; MU-RMS-40.16A; PPP1R45; PRKA9; repro12; YOTIAO
UniProt ID: (Human) Q99996, (Mouse) Q70FJ1
Entrez Gene ID: (Human) 10142, (Mouse) 100986
signal transduction
negative regulation of adenylate cyclase activity
spermatogenesis
Sertoli cell development
microtubule nucleation
chemical synaptic transmission
positive regulation of microtubule polymerization
protein complex localization
response to electrical stimulus
maintenance of centrosome location
regulation of membrane repolarization
regulation of ventricular cardiac muscle cell membrane repolarization
cellular response to cAMP
regulation of heart rate by cardiac conduction
regulation of cardiac muscle cell action potential involved in regulation of contraction
regulation of Golgi organization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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