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Invitrogen
ErbB4 Monoclonal Antibody (HFR1)
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FIGURE: 1 / 16
ErbB4 Antibody (MA1-861) in ICC/IF
Immunofluorescent analysis of ErbB4 (green) showing staining in the cytoplasm of SK-BR-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an ErbB4 monoclonal antibody (Product # MA1-861) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjug... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-861
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
HFR1
Immunogen
Synthetic Peptide: C R(1250) S T L Q H P D Y L Q E Y S T(1264)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-861 detects Her-4 from human and mouse samples.
MA1-861 has been successfully used in immunoprecipitation, immunohistochemical, and Western blotting procedures. By Western blot, this antibody detects ~185 kDa protein representing Her-4 from human A431 cell and mouse NIH3T3/HER4 cell extracts.This antibody has also been used in immunohistochemical, using paraffin embedded mouse NIH3T3 cells.
MA1-861 immunizing peptide corresponds to amino acid residues 1250 to 1264 of human Her-4 oncoprotein.
Target Information
Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: avian erythroblastosis oncogene B 4; OTTHUMP00000209432; p180erbB4; Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine-protein kinase erbB-4; Tyrosine kinase-type cell surface receptor HER4
Gene Aliases: c-erbB-4; ERBB4; HER4; Mer4
UniProt ID: (Mouse) Q61527
Entrez Gene ID: (Mouse) 13869
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
epidermal growth factor receptor binding
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein homodimerization activity
transcription regulatory region DNA binding
transmembrane signal receptor
neural crest cell migration
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
apoptotic process
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
nervous system development
heart development
lactation
positive regulation of cell proliferation
negative regulation of cell proliferation
embryonic pattern specification
negative regulation of muscle cell apoptotic process
phosphorylation
cell migration
peptidyl-tyrosine phosphorylation
central nervous system morphogenesis
olfactory bulb interneuron differentiation
regulation of cell migration
positive regulation of cell migration
positive regulation of synaptic transmission, GABAergic
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of apoptotic process
surfactant homeostasis
mitochondrial fragmentation involved in apoptotic process
cell fate commitment
positive regulation of transcription, DNA-templated
positive regulation of glucose import
protein autophosphorylation
positive regulation of epithelial cell proliferation
positive regulation of cardiac muscle cell proliferation
synapse maturation
mammary gland epithelial cell differentiation
mammary gland alveolus development
cardiac muscle tissue regeneration
positive regulation of ERK1 and ERK2 cascade
positive regulation of STAT protein import into nucleus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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