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Invitrogen
RAB9 Monoclonal Antibody (Mab9)
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FIGURE: 1 / 15
RAB9 Antibody (MA3-067) in ICC/IF
Immunofluorescence analysis of RAB9 was performed using HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAB9 Mouse Monoclonal Antibody (Product # MA3-067) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 for... View More
Product Details
MA3-067
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Hamster,
Human,
Non-human primate,
Rat
Published species
Hamster,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
Mab9
Immunogen
Recombinant canine rab 9a.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-067 recognizes prenylated and non-prenylated rab 9 from canine, human, monkey, rat, hamster and bovine tissues. This antibody does not cross-react with other rab family members.
MA3-067 has been successfully used in Western blot, immunofluorescence, EM immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~26 kDa protein representing rab 9 from K562 cell extract.
The MA3-067 antigen is recombinant canine rab 9a.
Target Information
Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: rab-9A; rab9; rab9 GTP-binding protein; RAB9, member RAS oncogene family; RAB9-like protein; RAB9A; RAB9A, member RAS oncogene family; Ras-related protein Rab-9A; unnamed protein product
Gene Aliases: RAB9; RAB9A
UniProt ID: (Dog) P24408, (Human) P51151, (Rat) Q99P75
Entrez Gene ID: (Bovine) 511776, (Dog) 403947, (Human) 9367, (Rat) 84589, (Chinese hamster) 100767493
protein transport
Rab protein signal transduction
regulation of protein localization
positive regulation of exocytosis
negative regulation by host of symbiont molecular function
receptor-mediated endocytosis
retrograde transport, endosome to Golgi
intracellular protein transport
metabolic process
phagosome-lysosome fusion
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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