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Invitrogen
HSP90 alpha Monoclonal Antibody (3B6)
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FIGURE: 1 / 18
HSP90 alpha Antibody (MA3-010) in ICC/IF
Immunofluorescence analysis of HSP90 alpha was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSP90 alpha Monoclonal Antibody(3B6) (Product # MA3-010) at 1:100 dilution in 0.1% BSA and incubated overnight ... View More
Product Details
MA3-010
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Bovine,
Human,
Mouse,
Non-human primate,
Rabbit
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
3B6
Immunogen
Purified mouse HSP90.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites, PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-010 detects heat shock protein 90 kDa (HSP90) from human, mouse, rat, and bovine tissues. This antibody does not detect chicken HSP90.
MA3-010 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects a 90 kDa protein representing HSP90 from MCF7 cell extract. MA3-011 is more sensitive in Western blot procedures and should be used when maximum sensitivity is desired. MA3-010 does not immunoprecipitate HSP90 in any form (free or complexed).
The MA3-010 antigen is purified mouse HSP90.
Target Information
Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; Heat shock protein; heat shock protein 1, alpha; heat shock protein 86; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; Heat shock protein family C member 1; Heat shock protein HSP 90-alpha; heat shock protein, 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; Heat shock protein90; HSP; HSP 86; HSP 90 alpha; HSP86; LAP-2; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; TSTA; Tumor-specific transplantation 86 kDa antigen; unnamed protein product
Gene Aliases: 86kDa; 89kDa; AL024080; AL024147; EL52; HEL-S-65p; Hsp103; hsp4; HSP86; Hsp86-1; Hsp89; HSP89A; Hsp90; HSP90A; HSP90AA1; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2
UniProt ID: (Bovine) Q76LV2, (Human) P07900, (Mouse) P07901, (Rat) P82995
Entrez Gene ID: (Bovine) 281832, (Human) 3320, (Mouse) 15519, (Rat) 299331
protein binding
ATP binding
ATPase activity
nitric-oxide synthase regulator activity
TPR domain binding
protein homodimerization activity
disordered domain specific binding
nucleotide binding
UTP binding
CTP binding
RNA binding
mRNA binding
GTP binding
hydrolase activity
sulfonylurea receptor binding
protein phosphatase binding
MHC class II protein complex binding
ubiquitin protein ligase binding
dATP binding
identical protein binding
histone deacetylase binding
protein binding involved in protein folding
ion channel binding
tau protein binding
GTPase binding
Rho GDP-dissociation inhibitor binding
unfolded protein binding
DNA polymerase binding
scaffold protein binding
ATP-dependent protein folding chaperone
enzyme-substrate adaptor activity
protein tyrosine kinase binding
protein kinase binding
poly(A) RNA binding
Hsp90 family chaperone
response to heat
response to cold
positive regulation of nitric oxide biosynthetic process
response to antibiotic
regulation of catalytic activity
neuron migration
activation of innate immune response
positive regulation of defense response to virus by host
skeletal muscle contraction
protein folding
mitochondrial transport
response to unfolded protein
telomere maintenance via telomerase
response to xenobiotic stimulus
response to salt stress
positive regulation of lamellipodium assembly
cardiac muscle cell apoptotic process
protein import into mitochondrial matrix
regulation of protein ubiquitination
positive regulation of telomere maintenance via telomerase
positive regulation of protein polymerization
positive regulation of interferon-beta production
regulation of protein localization
cellular response to heat
protein refolding
response to cocaine
positive regulation of protein import into nucleus
regulation of apoptotic process
regulation of protein complex assembly
protein unfolding
response to estrogen
positive regulation of protein catabolic process
positive regulation of cell size
nitric oxide metabolic process
protein stabilization
chaperone-mediated protein complex assembly
positive regulation of cardiac muscle contraction
chaperone-mediated autophagy
cellular response to virus
regulation of postsynaptic membrane neurotransmitter receptor levels
neurofibrillary tangle assembly
telomerase holoenzyme complex assembly
nitric oxide biosynthetic process
response to stress
positive regulation of protein import into nucleus, translocation
protein import into mitochondrial outer membrane
positive regulation of cytotoxic T cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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