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Invitrogen
mtHSP70 Monoclonal Antibody (JG1)
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.jpg?time=20240507?time=20240507 "mtHSP70 Antibody (MA3-028) in WB")
.jpg?time=20240507?time=20240507 "mtHSP70 Antibody (MA3-028) in WB")
Product Details
MA3-028
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Non-human primate
Published species
Dog,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
JG1
Immunogen
Synthetic peptide corresponding to residues G(615) S G S S G T G E Q K E D Q K E E K Q(633) of mouse mtHSP70.
Conjugate
Form
Liquid
Concentration
1.07 mg/mL
Amount
107 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-028 detects mitochondrial heat shock protein 70 kDa (mtHSP70) from human, non-human primate, canine and mouse tissues.
MA3-028 has been successfully used in Western blot, immunocytochemical, immunofluorescence, immunohistochemical (paraffin), and immunoprecipitation procedures. By Western blot, this antibody detects a single ~75 kDa band representing mtHSP70 from U2OS cell homogenate. Immunocytochemical staining of mtHSP70 in DAP.3 cells with MA3-028 results in a worm-like staining pattern, consistent with mitochondrial localization.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
Target Information
The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein which is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP78, or BiP, is a glucose-regulated 78 kDa protein localized to the endoplasmic reticulum; and mitochondrial HSP70 (mtHSP70, GRP75 or mortalin) a 75 kDa protein that is found within the mitochondria. mtHSP70 is a mitochondrial resident protein that is involved in protein translocation into the mitochondria. Preproteins cross the mitochondrial membranes in an extended conformation. This requires unfolding of preproteins before entering translocation pores in the mitochondrial outer membrane. Preprotein unfolding is thought to be mediated by mtHSP70 and other inner membrane proteins of the mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 68 kDa heat shock protein; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; Heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; Heat shock protein family A member 1A; Heat shock protein family A member 1B; heat shock protein, 70 kDa 3; heat shock-induced protein; Hsp68; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70.1/HSP70.2; HSP70.3; inducible heat shock protein 70; unnamed protein product
Gene Aliases: HEL-S-103; hsp68; HSP70; HSP70-1; HSP70-1A; HSP70-2; Hsp70-3; HSP70.1; HSP70.2; Hsp70.3; Hsp70A1; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSX70
UniProt ID: (Human) P0DMV8, (Mouse) Q61696
Entrez Gene ID: (Human) 3303, (Mouse) 193740
nucleotide binding
virus receptor activity
G-protein coupled receptor binding
transcription corepressor activity
RNA binding
receptor binding
protein binding
ATP binding
ATPase activity
enzyme binding
heat shock protein binding
denatured protein binding
ubiquitin protein ligase binding
death receptor agonist activity
histone deacetylase binding
protein binding involved in protein folding
cadherin binding
receptor agonist activity
unfolded protein binding
misfolded protein binding
C3HC4-type RING finger domain binding
disordered domain specific binding
transcription regulator inhibitor activity
ATP-dependent protein disaggregase activity
ATP-dependent protein folding chaperone
protease binding
double-stranded RNA binding
ATPase activity, coupled
poly(A) RNA binding
protein N-terminus binding
NF-kappaB binding
negative regulation of transcription from RNA polymerase II promoter
mRNA catabolic process
protein folding
response to unfolded protein
lysosomal transport
negative regulation of cell proliferation
positive regulation of gene expression
negative regulation of cell growth
negative regulation of transforming growth factor beta receptor signaling pathway
endoplasmic reticulum unfolded protein response
regulation of protein ubiquitination
negative regulation of protein ubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of interleukin-8 production
positive regulation of RNA splicing
cellular response to oxidative stress
cellular response to heat
cellular response to unfolded protein
protein refolding
negative regulation of apoptotic process
positive regulation of erythrocyte differentiation
ATP metabolic process
viral entry into host cell
protein stabilization
positive regulation of NF-kappaB transcription factor activity
chaperone-mediated protein complex assembly
cellular heat acclimation
positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway
cellular response to steroid hormone stimulus
positive regulation of microtubule nucleation
negative regulation of inclusion body assembly
negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
regulation of mitotic spindle assembly
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of tumor necrosis factor-mediated signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
telomere maintenance
DNA repair
response to heat
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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