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Invitrogen
ARF1/ARF3/ARF5/ARF6 Monoclonal Antibody (1D9)
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FIGURE: 1 / 15
ARF1/ARF3/ARF5/ARF6 Antibody (MA3-060) in ICC/IF
Immunofluorescent analysis of ADP-Ribosylation Factor using ADP-Ribosylation Factor Monoclonal Antibody (1D9) (Product # MA3-060) shows staining in Hela Cells. ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody re... View More
Product Details
MA3-060
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Hamster,
Human,
Mouse,
Rat
Published species
Bovine,
Hamster,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1D9
Immunogen
Recombinant human ARF1.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-060 detects ADP-ribosylation factor1 (Arf1), Arf3, Arf5 and Arf6 and about ten-fold less well with Arf4 from human, mouse and rat tissues.
MA3-060 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a single ~21 kDa protein representing Arf in rat pancreas extract. Immunofluorescence staining of Arf in rat pancreas with MA3-060 results in staining of the cytoplasmic face of the trans-Golgi membranes. MA3-060 cannot be used to neutralize Arf in solution.
The MA3-060 antigen is recombinant human Arf1.
Target Information
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ADP ribosylation factor 1; ADP ribosylation factor 3; ADP ribosylation factor 4; ADP ribosylation factor 5; ADP ribosylation factor 6; ADP-ribosylation factor (ARF1); ADP-ribosylation factor 1; ADP-ribosylation factor 2; ADP-ribosylation factor 3; ADP-ribosylation factor 4; ADP-ribosylation factor 4-like protein; ADP-ribosylation factor 5; ADP-ribosylation factor 6; ARF; ARF6; Liver regeneration-related protein LRRG202; pan ARF; small GTP binding protein; unnamed protein product
Gene Aliases: 5430400P17Rik; AA407803; Ac1-253; AI788669; AI854770; ARF1; ARF2; ARF3; ARF4; ARF5; ARF6; AW496366; I79_003112; PVNH8
UniProt ID: (Human) P84077, (Human) P61204, (Human) P18085, (Human) P84085, (Human) P62330, (Mouse) P84078, (Rat) P84079, (Rat) P84082, (Mouse) Q8BSL7, (Mouse) P61205, (Rat) P61206, (Rat) P61751, (Mouse) P61750, (Mouse) P84084, (Rat) P84083, (Mouse) P62331, (Rat) P62332
Entrez Gene ID: (Dog) 100856517, (Human) 375, (Human) 377, (Dog) 486562, (Dog) 607073, (Human) 378, (Human) 381, (Dog) 475206, (Dog) 490679, (Human) 382, (Chinese hamster) 100757864, (Mouse) 11840, (Rat) 64310, (Rat) 79119, (Mouse) 11841, (Mouse) 11842, (Chinese hamster) 100766631, (Rat) 140940, (Chinese hamster) 100769922, (Rat) 79120, (Mouse) 11843, (Mouse) 11844, (Chinese hamster) 100759676, (Rat) 79117, (Mouse) 11845, (Chinese hamster) 100774654, (Rat) 79121
nucleotide binding
magnesium ion binding
RNA binding
GTPase activity
protein binding
GTP binding
hydrolase activity
protein domain specific binding
guanyl-nucleotide exchange factor activity
epidermal growth factor receptor binding
NAD+-protein-arginine ADP-ribosyltransferase activity
phospholipase D activator activity
obsolete small monomeric GTPase activity
GDP binding
thioesterase binding
signaling adaptor activity
protein homodimerization activity
poly(A) RNA binding
protein N-terminus binding
G-protein
regulation of receptor internalization
cellular copper ion homeostasis
intracellular protein transport
protein transport
vesicle-mediated transport
regulation of Arp2/3 complex-mediated actin nucleation
long term synaptic depression
dendritic spine organization
cellular response to virus
mitotic cleavage furrow ingression
retrograde vesicle-mediated transport, Golgi to ER
ER to Golgi vesicle-mediated transport
epidermal growth factor receptor signaling pathway
learning
cell migration
negative regulation of apoptotic process
apical protein localization
establishment or maintenance of epithelial cell apical/basal polarity
positive regulation of transcription from RNA polymerase II promoter
response to axon injury
regulation of synapse organization
cilium morphogenesis
dendritic spine development
protein localization to cilium
regulation of postsynapse organization
regulation of cilium assembly
regulation of reactive oxygen species metabolic process
mitotic cytokinesis
liver development
vesicle docking involved in exocytosis
cell adhesion
nervous system development
regulation of neuron projection development
positive regulation of neuron projection development
cell differentiation
positive regulation of actin filament polymerization
cortical actin cytoskeleton organization
endocytic recycling
protein localization to cell surface
regulation of Rac protein signal transduction
protein localization to endosome
negative regulation of receptor-mediated endocytosis
synaptic vesicle endocytosis
positive regulation of protein secretion
cell division
regulation of filopodium assembly
positive regulation of keratinocyte migration
regulation of dendritic spine development
cellular response to diacyl bacterial lipopeptide
protein localization to plasma membrane
establishment of epithelial cell polarity
ruffle assembly
hepatocyte apoptotic process
maintenance of postsynaptic density structure
positive regulation of focal adhesion disassembly
erythrocyte apoptotic process
positive regulation of protein localization to plasma membrane
positive regulation of mitotic cytokinetic process
protein localization to cleavage furrow
regulation of presynapse assembly
cellular response to nerve growth factor stimulus
negative regulation of protein localization to cell surface
negative regulation of dendrite development
transport
actin filament organization
small GTPase mediated signal transduction
very-low-density lipoprotein particle assembly
positive regulation of catalytic activity
positive regulation of endocytosis
positive regulation of calcium ion-dependent exocytosis
Golgi to transport vesicle transport
positive regulation of dendritic spine development
synaptic vesicle budding
lysosomal membrane organization
positive regulation of sodium ion transmembrane transport
positive regulation of late endosome to lysosome transport
positive regulation of ER to Golgi vesicle-mediated transport
regulation of phospholipid metabolic process
protein ADP-ribosylation
brain development
activation of phospholipase D activity
cell cycle
ruffle organization
myeloid cell apoptotic process
regulation of toll-like receptor 4 signaling pathway
positive regulation of establishment of protein localization to plasma membrane
actin cytoskeleton organization
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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