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Invitrogen
TAF10 Monoclonal Antibody (23TA-1H8)
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FIGURE: 1 / 3
TAF10 Antibody (MA3-069) in ICC/IF
IImmunofluorescent analysis of TAF10 in HepG2 cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a TAF10 mouse monoclonal antibody (Product # MA3-069) at a dilution of 1:2000 in blocking buffer for 1 hour at room temperature, and then incubated with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:10... View More
Product Details
MA3-069
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
23TA-1H8
Immunogen
Synthetic peptide coupled to ovalalbumin aa 1 to 20
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-069 detects TAF10 from human, rat, and non-human primate samples. MA3-069 does not detect the TAF10 in mouse samples
MA3-069 has been successfully used in Western blot, immunofluorescence and flow cytometry applications.
The MA3-069 immunogen is the synthetic peptide coupled to ovalalbumin aa 1 to aa 20.
Target Information
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: STAF28; TAF(II)30; TAF10; TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa; TAFII-30; TATA box binding protein (TBP)-associated factor, RNA polymerase II, H, 30kD; transcription initiation factor TFIID 30 kD subunit; Transcription initiation factor TFIID 30 kDa subunit; Transcription initiation factor TFIID subunit 10
Gene Aliases: TAF10; TAF2A; TAF2H; TAFII30
UniProt ID: (Human) Q12962
Entrez Gene ID: (Human) 6881, (Rat) 293345
DNA binding
histone acetyltransferase activity
protein binding
obsolete general RNA polymerase II transcription factor activity
enzyme binding
estrogen receptor binding
identical protein binding
RNA polymerase binding
promoter-specific chromatin binding
molecular_function
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
general transcription factor
G1/S transition of mitotic cell cycle
in utero embryonic development
somitogenesis
liver development
embryonic placenta development
regulation of DNA repair
chromatin remodeling
DNA-templated transcription, initiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
apoptotic process
gene expression
regulation of gene expression
multicellular organism growth
SAGA complex assembly
mRNA transcription from RNA polymerase II promoter
regulation of RNA splicing
positive regulation of transcription, DNA-templated
lateral mesodermal cell differentiation
RNA polymerase II transcriptional preinitiation complex assembly
limb development
positive regulation of transcription initiation from RNA polymerase II promoter
protein-containing complex assembly
hepatocyte differentiation
allantois development
biological_process
histone deubiquitination
cellular protein complex assembly
histone H3 acetylation
regulation of DNA binding
protein homooligomerization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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