Search
Invitrogen
Ataxin 3 Monoclonal Antibody (2SCA-1H9)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 4
Ataxin 3 Antibody (MA3-082) in ICC/IF
Immunofluorescent analysis of Ataxin 3 (green) in Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Ataxin 3 mouse monoclonal antibody (Product # MA3-082) at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal™ Secondary Antibody, Alexa Fluor 488 conjugate (Product # A28... View More
Product Details
MA3-082
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2SCA-1H9
Immunogen
Human Ataxin-3 fragment from aa F112-aa L249
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-082 detects Ataxin 3 from human, mouse and rat samples.
MA3-082 has been successfully used in Western blot, immunofluorescence, and ELISA applications.
MA3-082 has been successfully used in Western blot, immunofluorescence, and ELISA applications. The MA3-082 immunogen is the human ataxin-3 fragment from aa 112 - aa L249 as a fusion protein.
Target Information
Machado-Joseph disease is an autosomal dominant neurologic disorder, and is now known to be the same as previously described spinocerebellar ataxia-3. MJD protein (Ataxin-3) contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. This protein interacts with key regulators (CBP, p300 and PCAF) of transcription and represses transcription, and also acts as a histone-binding protein that regulates transcription. MJD is a deubiquitinating enzyme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Ataxin-3; ataxin3; josephin; Machado-Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3) homolog; Machado-Joseph disease protein 1; Machado-Joseph disease protein 1 homolog; olivopontocerebellar ataxia 3; spin; Spinocerebellar ataxia type 3 protein
Gene Aliases: 2210008M02Rik; AI463012; AI647473; AT3; ataxin-3; ATX3; ATXN3; JOS; MJD; MJD1; Rsca3; SCA3
UniProt ID: (Human) P54252, (Rat) O35815, (Mouse) Q9CVD2
Entrez Gene ID: (Human) 4287, (Rat) 60331, (Mouse) 110616
thiol-dependent ubiquitin-specific protease activity
protein binding
peptidase activity
cysteine-type peptidase activity
hydrolase activity
ubiquitin protein ligase binding
identical protein binding
ATPase binding
Lys63-specific deubiquitinase activity
Lys48-specific deubiquitinase activity
RNA polymerase II regulatory region DNA binding
histone deacetylase activity
omega peptidase activity
chromatin/chromatin-binding, or -regulatory protein
microtubule cytoskeleton organization
cytoplasmic translation
nucleotide-excision repair
proteolysis
ubiquitin-dependent protein catabolic process
misfolded or incompletely synthesized protein catabolic process
chemical synaptic transmission
nervous system development
cellular response to starvation
regulation of cell-substrate adhesion
protein deubiquitination
actin cytoskeleton organization
cellular response to nutrient levels
cellular response to amino acid starvation
cellular response to heat
monoubiquitinated protein deubiquitination
exploration behavior
TORC1 signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
intermediate filament cytoskeleton organization
negative regulation of translational initiation
positive regulation of translational initiation
protein K63-linked deubiquitination
protein K48-linked deubiquitination
cellular response to misfolded protein
negative regulation of TORC1 signaling
positive regulation of TORC1 signaling
positive regulation of ERAD pathway
protein localization to cytosolic proteasome complex
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
transcription, DNA-templated
regulation of transcription, DNA-templated
histone H3 deacetylation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support