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Invitrogen
SERCA1 ATPase Monoclonal Antibody (IIH11)
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FIGURE: 1 / 25
SERCA1 ATPase Antibody (MA3-911) in ICC/IF
Immunofluorescent analysis of SERCA1 ATPase using Anti-SERCA1 ATPase Monoclonal Antibody (IIH11) (Product # MA3-911) shows staining in C6 Cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SER... View More
Product Details
MA3-911
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Guinea pig,
Human,
Mouse,
Rabbit,
Rat
Published species
Dog,
Human,
Invertebrate,
Mouse,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
IIH11
Immunogen
Purified rabbit skeletal muscle sarcoplasmic reticulum.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
PBS, ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-911 detects sarcoplasmic or endoplasmic reticulum calcium 1 (SERCA1) ATPase from canine, human, rabbit, rat, mouse and guinea pig tissues.
MA3-911 has been successfully used in Western blot, immunohistochemistry, and immunofluorescence procedures. By Western blot, this antibody detects an ~110 kDa protein representing SERCA1 ATPase in canine skeletal muscle extracts. Immunofluorescence staining of SERCA1 ATPase in canine skeletal muscle with MA3-911 results in strong labeling of the entire type II (fast) myofiber.
The MA3-911 antigen is purified rabbit skeletal muscle sarcoplasmic reticulum. This antibody recognizes an epitope between amino acids 199-505 of rabbit skeletal muscle ATPase, a region that is not exposed in native sarcoplasmic reticulum.
Target Information
ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: adult Ca2+ ATPase; ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; ATPase, Ca++ transporting, ubiquitous; Ca2+ ATPase; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; neonatal Ca2+ ATPase; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA; SERCA ATPase; SERCA1; SR Ca(2+)-ATPase 1; unnamed protein product
Gene Aliases: ATP2A; ATP2A1; ATP2A3; SERCA1; SERCA1a
UniProt ID: (Human) O14983, (Rabbit) P04191, (Mouse) Q8R429, (Rat) Q64578
Entrez Gene ID: (Dog) 479797, (Human) 487, (Rabbit) 100037716, (Mouse) 11937, (Rat) 116601, (Guinea pig) 100726234
nucleotide binding
calcium-transporting ATPase activity
calcium ion binding
protein binding
ATP binding
ATPase activity
calcium-dependent ATPase activity
protein homodimerization activity
metal ion binding
nucleotide-sugar transmembrane transporter activity
hydrolase activity
primary active transporter
ion transport
calcium ion transport
cellular calcium ion homeostasis
regulation of striated muscle contraction
apoptotic mitochondrial changes
positive regulation of fast-twitch skeletal muscle fiber contraction
positive regulation of endoplasmic reticulum calcium ion concentration
negative regulation of endoplasmic reticulum calcium ion concentration
ion transmembrane transport
response to endoplasmic reticulum stress
negative regulation of striated muscle contraction
positive regulation of mitochondrial calcium ion concentration
maintenance of mitochondrion location
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
calcium ion import
calcium ion transmembrane transport
relaxation of skeletal muscle
positive regulation of cardiac muscle cell contraction
positive regulation of calcium-transporting ATPase activity
positive regulation of calcium ion import into sarcoplasmic reticulum
regulation of cardiac conduction
calcium ion import into sarcoplasmic reticulum
transport
regulation of muscle contraction
response to peptide hormone
sarcoplasmic reticulum calcium ion transport
carbohydrate derivative transport
nucleotide transmembrane transport
metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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