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Invitrogen
Calsequestrin Monoclonal Antibody (VIIID12)
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FIGURE: 1 / 45
Calsequestrin Antibody (MA3-913) in ICC/IF
Immunofluorescent analysis of Calsequestrin using Anti-Calsequestrin Monoclonal Antibody (VIIID12) (Product # MA3-913) shows staining in MCF-7 Cells. Calsequestrin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizin... View More
Product Details
MA3-913
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Human,
Mouse,
Pig,
Rabbit,
Rat
Published species
Chicken,
Dog,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Rodent
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
VIIID12
Immunogen
Purified rabbit skeletal muscle sarcoplasmic reticulum.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
PBS, ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-913 detects calsequestrin from human, mouse, rat, canine, porcine, chicken, and rabbit skeletal muscle tissues. This antibody recognizes calsequestrin in both type I (slow) and type II (fast) skeletal muscle tissues.
MA3-913 has been successfully used in Western blot procedures. By Western blot, this antibody detects a 63 kDa protein representing calsequestrin from canine skeletal muscle extracts. Higher molecular weight proteins are seen on the Western blot and are believed to be calsequestrin-like proteins found in the sarcoplasmic reticulum. The staining pattern yields double rows of fluorescent dots corresponding to triad pairs on either side of the Z-line.
The MA3-913 antigen is purified rabbit skeletal muscle sarcoplasmic reticulum.
Target Information
The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Aspartactin; calmitin; Calmitine; calsequestrin; calsequestrin 1 (fast-twitch, skeletal muscle); Calsequestrin, skeletal muscle isoform; Calsequestrin-1; calsquestrin 1; Laminin-binding protein; mitochondrial calcium-binding protein; skeletal muscle calsequestrin 1; unnamed protein product
Gene Aliases: CASQ; CASQ1; CSQ; CSQ-1; CSQ1; PDIB1; sCSQ; VMCQA
UniProt ID: (Rabbit) P07221, (Human) P31415, (Rat) P19633, (Mouse) O09165
Entrez Gene ID: (Rabbit) 100009095, (Pig) 100156448, (Dog) 608401, (Human) 844, (Rat) 686019, (Mouse) 12372
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of store-operated calcium channel activity
regulation of store-operated calcium entry
endoplasmic reticulum organization
skeletal muscle tissue development
response to heat
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion
response to muscle inactivity
response to denervation involved in regulation of muscle adaptation
sarcomere organization
protein polymerization
regulation of release of sequestered calcium ion into cytosol
regulation of sequestering of calcium ion
response to organic substance
regulation of muscle contraction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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