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Invitrogen
ATP1A3 Monoclonal Antibody (XVIF9-G10)
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FIGURE: 1 / 55
ATP1A3 Antibody (MA3-915) in ICC/IF
Immunofluorescence analysis of ATP1A3 was performed using SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ATP1A3 Monoclonal Antibody (XVIF9-G10) (Product # MA3-915) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723), (1:2000), for ... View More
Product Details
MA3-915
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Guinea pig,
Human,
Mouse,
Non-human primate,
Sheep,
Rabbit,
Rat
Published species
Amphibian,
Bovine,
Gerbil,
Guinea pig,
Human,
Mouse,
Non-human primate,
Pig,
Rat,
Shark
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
XVIF9-G10
Immunogen
Canine cardiac microsomes.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-915 detects sodium/potassium ATPase from human, monkey, bovine, sheep, canine, rabbit, guinea pig, mouse and rat tissue. This antibody is specific for the alpha-3 subunit.
MA3-915 has been successfully used in Western blot and immunohistochemical procedures. By Western blot, this antibody detects an ~110 kDa protein representing the alpha-3 subunit of the sodium/potassium ATPase from canine skeletal muscle extract. Immunohistochemical staining of sodium/potassium ATPase in rat retina with MA3-915 yields a pattern consistent with plasma membrane localization.
The MA3-915 antigen is canine cardiac microsomes.
Target Information
The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha-3 subunit has been found in neuronal and to skeletal and cardiac muscle, lung and stomach tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATPase, Na+/K+ transporting, alpha 3 polypeptide; ATPase, Na+K+ transporting, alpha 3 subunit; EC 3.6.3.9; Na(+)/K(+) ATPase alpha(III) subunit; Na(+)/K(+) ATPase alpha-3 subunit; Na+, K+ activated adenosine triphosphatase alpha subunit; Na+/K+ -ATPase alpha 3 subunit; Na+/K+ ATPase 3; Sodium Potassium ATPase; Sodium pump 3; Sodium pump subunit alpha-3; sodium-potassium ATPase catalytic subunit alpha-3; sodium-potassium-ATPase, alpha 3 polypeptide; sodium/potassium-transporting ATPase alpha-3 chain; Sodium/potassium-transporting ATPase subunit alpha-3; unnamed protein product
Gene Aliases: AHC2; ATP1A1; ATP1A3; Atpa-2; ATPa1a3; CAPOS; DEE99; DYT12; RDP
UniProt ID: (Human) P13637, (Rat) P06687, (Mouse) Q6PIC6
Entrez Gene ID: (Sheep) 101103751, (Bovine) 282598, (Dog) 612463, (Human) 478, (Rabbit) 100349382, (Rat) 24213, (Guinea pig) 100712985, (Mouse) 232975
nucleotide binding
beta-amyloid binding
sodium:potassium-exchanging ATPase activity
protein binding
ATP binding
potassium-transporting ATPase activity
ATPase activity
metal ion binding
chaperone binding
steroid hormone binding
cation-transporting ATPase activity
D1 dopamine receptor binding
hydrolase activity
heparan sulfate proteoglycan binding
sodium:potassium-exchanging ATPase activity involved in regulation of cardiac muscle cell membrane potential
primary active transporter
ion transport
potassium ion transport
sodium ion transport
cellular sodium ion homeostasis
establishment or maintenance of transmembrane electrochemical gradient
cellular cation homeostasis
cellular potassium ion homeostasis
sodium ion export from cell
regulation of resting membrane potential
cellular response to steroid hormone stimulus
cell communication by electrical coupling involved in cardiac conduction
hydrogen ion transmembrane transport
response to glycoside
cellular response to beta-amyloid
neuron projection maintenance
potassium ion import across plasma membrane
memory
metabolic process
adult locomotory behavior
visual learning
potassium ion import
ATP hydrolysis coupled proton transport
ionotropic glutamate receptor signaling pathway
response to drug
transport
sodium ion transmembrane transport
cardiac muscle contraction
potassium ion transmembrane transport
regulation of cardiac muscle cell membrane potential
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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