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Invitrogen
ATP4B Monoclonal Antibody (2G11)
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FIGURE: 1 / 22
ATP4B Antibody (MA3-923) in ICC/IF
Immunofluorescent analysis of Hydrogen/Potassium ATPase beta using Anti-Hydrogen/Potassium ATPase beta Monoclonal Antibody (2G11) (Product # MA3-923) shows staining in A2058 Cells. Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta (Product # MA3-923) at a dilution of 1:100 over night ... View More
Product Details
MA3-923
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Human,
Mustelid,
Mouse,
Pig,
Rabbit,
Rat
Published species
Bovine,
Guinea pig,
Human,
Mouse,
Rabbit,
Rat,
Rodent
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2G11
Immunogen
Purified 34 kDa core peptide from deglycosylated hog gastric microsomes.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
PBS, ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-923 detects the beta-subunit of hydrogen/potassium ATPase from bovine, human, canine, porcine, rabbit, mouse, ferret, and rat tissues.
MA3-923 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation procedures (ChIP). By Western blot, this antibody detects various forms of the beta-subunit, including a 60 to 80 kDa glycosylated protein, a 52 kDa beta-subunit precursor, and the 34 kDa core peptide from rabbit stomach extracts. Immunohistochemical staining of hydrogen/potassium ATPase in rabbit gastric glands with MA3-923 results in specific staining of parietal cells.
MA3-923 has been shown to inhibit the enzymatic activity of the hydrogen/potassium ATPase and to alter the affinity of the cytoplasmic potassium binding site.
The MA3-923 antigen is purified 34 kDa core peptide from deglycosylated hog gastric microsomes. This antibody recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cytoplasmic side of the beta-subunit.
Target Information
The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation-transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha- subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATPase H+/K+ transporting beta subunit; ATPase H+K+-transporting beta / (gastric HK-ATPase beta subunit) defined by SSR; ATPase, H+,K+-transporting, beta / (gastric H,K-ATPase beta subunit), defined by SSR; ATPase, H+/K+ exchanging, beta polypeptide; ATPase, H+/K+ transporting, beta polypeptide; ATPase, H+/K+ transporting, beta polypeptide, gastric specific; Gastric H(+)/K(+) ATPase subunit beta; gastric H+/K+ ATPase beta subunit; gastric H+/K+-ATPase beta subunit; gastric hydrogen-potassium ATPase, beta; gp60-90; H+,K+-ATPase; H+/K+ ATPase beta; H+/K+ ATPase beta subunit; H+/K+-ATPase beta; H+/K+-ATPase beta subunit; H,K-ATPase-Beta; H.K-ATPase, beta subunit; potassium-transporting ATPase beta chain; Potassium-transporting ATPase subunit beta; Proton pump beta chain
Gene Aliases: ATP4B; ATP6B; AV080843; S76401S1
UniProt ID: (Pig) P18434, (Rabbit) P18597, (Human) P51164, (Dog) P33704, (Rat) P18598, (Mouse) P50992
Entrez Gene ID: (Pig) 397131, (Rabbit) 100009125, (Human) 496, (Bovine) 614308, (Dog) 404020, (Rat) 24217, (Mouse) 11945
cell adhesion
establishment or maintenance of transmembrane electrochemical gradient
positive regulation of ATPase activity
ion transport
potassium ion transport
sodium ion transport
cellular sodium ion homeostasis
cellular potassium ion homeostasis
response to lipopolysaccharide
sodium ion export from cell
pH reduction
potassium ion transmembrane transport
hydrogen ion transmembrane transport
potassium ion import across plasma membrane
gastric acid secretion
response to organonitrogen compound
transport
regulation of proton transport
proton transport
protein glycosylation in endoplasmic reticulum
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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