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Invitrogen
ATP1B1 Monoclonal Antibody (M17-P5-F11)
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FIGURE: 1 / 34
ATP1B1 Antibody (MA3-930) in ICC/IF
Immunofluorescent analysis of Sodiµm/Potassium ATPase beta using Anti-Sodiµm/Potassium ATPase beta Monoclonal Antibody (M17-P5-F11) (Product # MA3-930) shows staining in MCF-7 Cells. Sodiµm/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodiµm/Potassium ATPase beta (Product # MA3-930) at a dilution of 1:200 over night at... View More
Product Details
MA3-930
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Sheep,
Pig
Published species
Dog,
Fruit fly,
Human,
Invertebrate,
Mouse,
Pig,
Sheep
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
M17-P5-F11
Immunogen
Purified lamb kidney beta sodium/potassium ATPase.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-930 detects sodium/potassium ATPase beta 1 from mouse, human, sheep, canine, and pig tissues. This antibody does not recognize the beta 1 or beta 2 subunit in rat.
MA3-930 has been successfully used in Western blot and immunohistochemistry and immunocytochemistry procedures. By Western blot, this antibody detects an ~42 kDa protein in liver samples and ~55 kDa in kidney samples representing beta sodium/potassium ATPase in sheep liver extract. Immunohistochemical staining of the sodium/potassium ATPase with MA3-930 yields a pattern consistent with plasma membrane localization.
The MA3-930 antigen is purified lamb kidney sodium/potassium ATPase beta. This antibody recognizes and epitope between amino acid residues 195-199 of sheep sodium/potassium ATPase beta 1.
Target Information
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: adenosinetriphosphatase; ATP1B1; ATPase beta subunit; ATPase, Na+/K+ transporting, beta 1 polypeptide; Beta 1-subunit of Na(+),K(+)-ATPase; MGC1798; Na+, K+-ATPase; Na+, K+-ATPase beta-subunit; Na+/K+ ATPase Beta; Na, K-ATPase beta-1 polypeptide; OTTHUMP00000032537; OTTHUMP00000032538; Sodium Potassium ATPase; sodium pump subunit beta-1; sodium-potassium ATPase subunit beta 1 (non-catalytic); sodium/potassium ATPase beta subunit; sodium/potassium-dependent ATPase beta-1 subunit; Sodium/potassium-dependent ATPase subunit beta-1; sodium/potassium-transporting ATPase beta-1 chain; Sodium/potassium-transporting ATPase subunit beta-1; unnamed protein product
Gene Aliases: ATP1B; ATP1B1; Atp4b; Atpb; Atpb-1; NKbeta1
UniProt ID: (Sheep) P05028, (Pig) P05027, (Human) P05026, (Mouse) P14094
Entrez Gene ID: (Sheep) 443384, (Dog) 403966, (Pig) 396898, (Human) 481, (Mouse) 11931
sodium:potassium-exchanging ATPase activity
protein binding
ATP binding
protein C-terminus binding
drug binding
ATPase activity
protein kinase binding
potassium ion binding
sodium ion binding
ATPase binding
ATPase activator activity
MHC class II protein complex binding
protein binding, bridging
protein heterodimerization activity
transporter activator activity
primary active transporter
cell adhesion
establishment or maintenance of transmembrane electrochemical gradient
positive regulation of ATPase activity
cellular calcium ion homeostasis
cellular sodium ion homeostasis
regulation of gene expression
regulation of cardiac muscle contraction by calcium ion signaling
cellular potassium ion homeostasis
sodium ion export from cell
ATP metabolic process
protein stabilization
transmembrane transport
relaxation of cardiac muscle
cardiac muscle contraction
protein localization to plasma membrane
membrane repolarization
positive regulation of potassium ion transmembrane transporter activity
positive regulation of sodium ion export from cell
positive regulation of calcium:sodium antiporter activity
positive regulation of potassium ion import
potassium ion import across plasma membrane
response to hypoxia
immune system process
ion transport
potassium ion transport
sodium ion transport
metal ion transport
sodium ion transmembrane transport
protein transport into plasma membrane raft
innate immune response
membrane repolarization during cardiac muscle cell action potential
cell communication by electrical coupling involved in cardiac conduction
hydrogen ion transmembrane transport
regulation of calcium ion transmembrane transport
blastocyst development
positive regulation of defense response to virus by host
transport
potassium ion import
mitophagy in response to mitochondrial depolarization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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