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Invitrogen
CD81 Monoclonal Antibody (1.3.3.22)
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FIGURE: 1 / 25
CD81 Antibody (MA5-13548) in ICC/IF
Immunofluorescent analysis of CD81 (green) showing staining in the membrane of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD81 monoclonal antibody (Product # MA5-13548) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjuga... View More
Product Details
MA5-13548
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
1.3.3.22
Immunogen
B cell line derived from a Burkitt lymphoma
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-13548 targets CD81 in WB, FACS, FN, ICC/IF, and IHC (F) applications and shows reactivity with Human and Rat samples.
The MA5-13548 immunogen is b cell line derived from a Burkitt lymphoma.
Target Information
CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 26 kDa cell surface protein TAPA-1; 26-kDa cell surface protein TAPA-1; CD 81 antigen; CD81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); Target of the antiproliferative antibody 1; Tetraspanin-28; Tetraspanin28; Tspan-28
Gene Aliases: CD81; CVID6; S5.7; TAPA1; TSPAN28
UniProt ID: (Human) P60033
Entrez Gene ID: (Human) 975, (Rat) 25621
activation of MAPK activity
phosphatidylinositol biosynthetic process
protein localization
positive regulation of cell proliferation
response to wounding
positive regulation of B cell proliferation
regulation of growth
regulation of cell proliferation
positive regulation of 1-phosphatidylinositol 4-kinase activity
phosphatidylinositol metabolic process
positive regulation of peptidyl-tyrosine phosphorylation
regulation of cell motility
immunological synapse formation
adaptive immune response
immune system process
humoral immune response mediated by circulating immunoglobulin
positive regulation of inflammatory response to antigenic stimulus
myoblast fusion involved in skeletal muscle regeneration
receptor internalization
regulation of protein stability
macrophage fusion
CD4-positive, alpha-beta T cell costimulation
positive regulation of MAPK cascade
positive regulation of transcription from RNA polymerase II promoter
viral entry into host cell
positive regulation of B cell receptor signaling pathway
positive regulation of T cell receptor signaling pathway
positive regulation of B cell activation
protein localization to lysosome
positive regulation of protein exit from endoplasmic reticulum
cellular response to low-density lipoprotein particle stimulus
protein localization to plasma membrane
osteoclast fusion
positive regulation of receptor clustering
positive regulation of protein catabolic process in the vacuole
regulation of macrophage migration
positive regulation of adaptive immune memory response
positive regulation of T-helper 2 cell cytokine production
positive regulation of CD4-positive, alpha-beta T cell proliferation
positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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