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Invitrogen
PCAF Monoclonal Antibody (E.107.0)
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FIGURE: 1 / 2
PCAF Antibody (MA5-11186) in WB
Western blot was performed using PCAF Monoclonal Antibody (E.107.0) (Product # MA5-11186) and a 93 kDa band corresponding to PCAF was observed in all the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), PC-3 (Lane 2), C2C12 (Lane 3), HEK-293 (Lane 4) and (45 µg lysate) of LNCAP (Lane 5) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was ... View More
Product Details
MA5-11186
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
E.107.0
Immunogen
Synthetic peptide corresponding to the amino terminus of human PCAF protein
Conjugate
Form
Liquid
Concentration
358 µg/mL
Amount
35.8 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with the related GCN5L2 protein.
Target Information
PCAF (histone acetyltransferase KAT2B) functions as a histone acetyltransferase (HAT) to promote transcriptional activation. It has significant histone acetyltransferase activity with core histones (H3 and H4) and nucleosome core particles. PCAF also acetylates non-histone proteins such as ACLY, PLK4, and TBX5. It inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. PCAF also acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It is involved in heart and limb development by mediating acetylation of TBX5 and acetylation regulating nucleocytoplasmic shutting of TBX5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CREBBP-associated factor; Histone acetylase PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; K(lysine) acetyltransferase 2B; Lysine acetyltransferase 2B; P/ PCAF; P/CAF; P300/CBP-associated factor; Spermidine acetyltransferase KAT2B
Gene Aliases: A930006P13Rik; AI461839; AW536563; CAF; KAT2B; P/CAF; PCAF
UniProt ID: (Human) Q92831, (Mouse) Q9JHD1
Entrez Gene ID: (Human) 8850, (Rat) 301164, (Mouse) 18519
RNA polymerase II regulatory region sequence-specific DNA binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
diamine N-acetyltransferase activity
histone acetyltransferase activity
lysine N-acetyltransferase activity, acting on acetyl phosphate as donor
cyclin-dependent protein serine/threonine kinase inhibitor activity
protein binding
enzyme activator activity
H3 histone acetyltransferase activity
acetyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
protein kinase binding
histone acetyltransferase binding
histone deacetylase binding
histone acetyltransferase activity (H3-K9 specific)
peptide-lysine-N-acetyltransferase activity
DNA-binding transcription factor binding
transcription factor binding
protein complex binding
N-acetyltransferase activity
chromatin remodeling
protein acetylation
memory
negative regulation of cell proliferation
regulation of protein ADP-ribosylation
positive regulation of neuron projection development
histone acetylation
N-terminal peptidyl-lysine acetylation
internal peptidyl-lysine acetylation
peptidyl-lysine acetylation
cellular response to insulin stimulus
cellular response to oxidative stress
positive regulation of chromatin binding
positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter
histone H3 acetylation
histone H3-K9 acetylation
negative regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of transcription, DNA-templated
positive regulation of vasodilation
positive regulation of transcription from RNA polymerase II promoter
cellular response to parathyroid hormone stimulus
positive regulation of histone H3-K14 acetylation
positive regulation of histone H3-K9 acetylation
negative regulation of transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter by glucose
gluconeogenesis
regulation of DNA repair
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
ubiquitin-dependent protein catabolic process
fatty acid biosynthetic process
heart development
regulation of gene expression
vasodilation
regulation of RNA splicing
negative regulation of fatty acid biosynthetic process
positive regulation of gluconeogenesis
positive regulation of fatty acid biosynthetic process
positive regulation of gene expression, epigenetic
positive regulation of glycolytic process
regulation of embryonic development
negative regulation of centriole replication
positive regulation of lipid biosynthetic process
rhythmic process
regulation of cell division
attachment of mitotic spindle microtubules to kinetochore
regulation of cell cycle
limb development
regulation of small molecule metabolic process
negative regulation of ferroptosis
positive regulation of attachment of mitotic spindle microtubules to kinetochore
negative regulation of rRNA processing
transcription, DNA-templated
cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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