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Invitrogen
CDK1 Monoclonal Antibody (A17.1.1)
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FIGURE: 1 / 19
CDK1 Antibody (MA5-11472) in ICC/IF
Immunofluorescent analysis of Cdk1 (green) showing staining in the cytoplasm of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cdk1 monoclonal antibody (Product # MA5-11472) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-acti... View More
Product Details
MA5-11472
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Guinea pig,
Human,
Mouse,
Rodent,
Rat,
Xenopus
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
A17.1.1
Immunogen
C-Terminal 2/3 of Xenopus CDC2 expressed in E. coli
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-11472 targets Cdk1 in ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Chicken, Guinea Pig, Human, mouse, Rodent, Rat, and Xenopus laevis samples.
The MA5-11472 immunogen is c-Terminal 2/3 of Xenopus CDC2 expressed in E. coli.
Target Information
CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ABL1; CDK1; CDK1-A; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; Cell division control protein 2 homolog; Cell division control protein 2 homolog 1; Cell division control protein 2-A; cell division cycle 2 homolog A; cell division cycle 2 like; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; Cell division protein kinase 1; Cell division protein kinase 1-A; Cyclin-dependent kinase 1; Cyclin-dependent kinase 1-A; DKFZp686L20222; KCIP-1; maturation-promoting factor; MGC111195; MGC53829; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase; p34 protein kinase 1; Protein 1054; unnamed protein product
Gene Aliases:
cdc-2; CDC2; cdc2-a; CDC28A; Cdc2a; cdc2x1.1; CDK1; cdk1-a; cdk1-b; CDKN1; MPF; p34
UniProt ID: (Chicken) P13863, (Human) P06493, (Rat) P39951, (Mouse) P11440, (Xenopus) P35567
Entrez Gene ID: (Chicken) 396252, (Human) 983, (Guinea pig) 100735946, (Rat) 54237, (Mouse) 12534, (Xenopus) 380246
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
nucleotide binding
virus receptor activity
chromatin binding
protein kinase activity
protein binding
kinase activity
transferase activity
cyclin binding
Hsp70 protein binding
histone kinase activity
cyclin-dependent protein kinase activity
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
G2/M transition of mitotic cell cycle
double-strand break repair via homologous recombination
protein phosphorylation
mitotic G2 DNA damage checkpoint
cellular response to heat
regulation of circadian rhythm
rhythmic process
cell division
G1/S transition of mitotic cell cycle
microtubule cytoskeleton organization
DNA replication
DNA repair
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
apoptotic process
cellular response to DNA damage stimulus
nuclear envelope organization
mitotic nuclear envelope disassembly
centrosome cycle
pronuclear fusion
response to toxic substance
positive regulation of gene expression
negative regulation of gene expression
positive regulation of G2/M transition of mitotic cell cycle
regulation of Schwann cell differentiation
response to amine
response to activity
cell migration
protein deubiquitination
peptidyl-threonine phosphorylation
chromosome condensation
epithelial cell differentiation
protein localization to kinetochore
positive regulation of protein import into nucleus
response to hydrogen peroxide
negative regulation of apoptotic process
mitotic cell cycle phase transition
response to ethanol
positive regulation of DNA replication
regulation of embryonic development
thymidine biosynthetic process
response to cadmium ion
response to copper ion
viral entry into host cell
microtubule polymerization
fibroblast proliferation
response to axon injury
ventricular cardiac muscle cell development
positive regulation of cardiac muscle cell proliferation
cilium disassembly
positive regulation of mitotic sister chromatid segregation
protein-containing complex assembly
cellular response to hydrogen peroxide
ERK1 and ERK2 cascade
Golgi disassembly
DNA strand resection involved in replication fork processing
positive regulation of protein localization to nucleus
regulation of mitotic attachment of spindle microtubules to kinetochore
microtubule cytoskeleton organization involved in mitosis
DNA synthesis involved in mitotic DNA replication
positive regulation of mitochondrial ATP synthesis coupled electron transport
protein localization to site of double-strand break
mitotic DNA-dependent DNA replication
mitotic cell cycle
protein complex assembly
mitotic nuclear division
cell aging
response to organonitrogen compound
response to organic cyclic compound
histone phosphorylation
peptidyl-serine phosphorylation
organ regeneration
positive regulation of protein import into nucleus, translocation
response to drug
positive regulation of mitotic cell cycle
cell cycle
phosphorylation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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