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Invitrogen
Caspase 3 Monoclonal Antibody (3CSP03 (4.1.18))
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FIGURE: 1 / 12
Caspase 3 Antibody (MA5-11521) in ICC/IF
Immunofluorescence analysis of Caspase 3 was performed using 70% confluent log phase HeLa cells treated with 1 µg/mL Staurosporine for 5 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Caspase 3 Monoclonal Antibody (3CSP03 (4.1.18)) (product # MA5-246... View More
Product Details
MA5-11521
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
3CSP03 (4.1.18)
Immunogen
Recombinant full length human Caspase 3 protein
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-11521 targets Caspase 3 in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Human samples.
The MA5-11521 immunogen is recombinant full length human Caspase 3 protein.
Target Information
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apopain; Apopain precursor; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase3; CPP-32; Cysteine protease CPP32; ICE3; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; unnamed protein product
Gene Aliases: CASP3; CPP32; CPP32B; SCA-1
UniProt ID: (Human) P42574
Entrez Gene ID: (Human) 836
protease binding
endopeptidase activity
aspartic-type endopeptidase activity
cysteine-type endopeptidase activity
cyclin-dependent protein serine/threonine kinase inhibitor activity
death receptor binding
protein binding
enzyme activator activity
peptidase activity
cysteine-type peptidase activity
phospholipase A2 activator activity
hydrolase activity
macromolecular complex binding
luteolysis
response to hypoxia
B cell homeostasis
negative regulation of cytokine production
proteolysis
apoptotic process
cellular response to DNA damage stimulus
axonal fasciculation
heart development
sensory perception of sound
learning or memory
intrinsic apoptotic signaling pathway in response to osmotic stress
response to xenobiotic stimulus
response to UV
response to wounding
response to glucose
response to metal ion
response to X-ray
regulation of macroautophagy
protein processing
hippocampus development
protein catabolic process
neuron differentiation
keratinocyte differentiation
erythrocyte differentiation
platelet formation
negative regulation of B cell proliferation
regulation of protein stability
response to cobalt ion
response to estradiol
response to lipopolysaccharide
glial cell apoptotic process
response to tumor necrosis factor
response to nicotine
intracellular signal transduction
interleukin-18-mediated signaling pathway
response to hydrogen peroxide
T cell homeostasis
positive regulation of apoptotic process
response to amino acid
positive regulation of neuron apoptotic process
fibroblast apoptotic process
cell fate commitment
negative regulation of cell cycle
negative regulation of activated T cell proliferation
neurotrophin TRK receptor signaling pathway
striated muscle cell differentiation
response to glucocorticoid
neuron apoptotic process
protein maturation
anterior neural tube closure
protein poly-ADP-ribosylation
pyroptosis
leukocyte apoptotic process
response to anesthetic
cellular response to staurosporine
apoptotic signaling pathway
intrinsic apoptotic signaling pathway
execution phase of apoptosis
regulation of synaptic vesicle cycle
positive regulation of pyroptotic inflammatory response
positive regulation of beta-amyloid formation
epithelial cell apoptotic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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