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Invitrogen
CD24 Monoclonal Antibody (SN3)
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FIGURE: 1 / 17
CD24 Antibody (MA5-11828) in ICC/IF
Immunofluorescent analysis of CD24 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD24 monoclonal antibody (Product # MA5-11828) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjug... View More
Product Details
MA5-11828
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
SN3
Immunogen
Cell membrane antigen preparation isolated from NALM-1 human pre-B leukemia cell line
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-11828 targets CD24 in FACS, IHC (F), ICC/IF and WB applications and shows reactivity with Human and Mouse samples. This antibody detects a few non-specific bands in BAF-3 cell lysates.
The MA5-11828 immunogen is cell membrane antigen preparation isolated from NALM-1 human pre-B leukemia cell line.
Target Information
CD24 is a 35-50 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on a variety of cell types, including erythrocytes, thymocytes, peripheral lymphocytes, and cells of the myeloid lineage. It is variably glycosylated, resulting in heterogeneity in molecular mass across different cell lineages, which can affect antibody staining levels on lymphocyte populations. In the context of B-cell development, CD24 is expressed from the pro-B-cell stage in the bone marrow through to mature, surface Ig-positive B cells, with very low or negative expression on plasma cells. It is also present on the majority of B-lineage acute lymphoblastic leukemias, B-cell chronic lymphocytic leukemias (CCLs), and B-cell non-Hodgkin's lymphomas. CD24 is thought to play a role in regulating B-cell proliferation and maturation, as well as in controlling autoimmunity. Additionally, CD24 has been reported to interact with P-selectin (CD62P), indicating a potential role in cell adhesion and signaling. CD24 is a valuable marker for studying B-cell development, leukemia, lymphoma, and autoimmune regulation, as well as its interactions with other molecules like P-selectin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); cluster of differentiation 24; FLJ22950; FLJ43543; heat stable antigen; HSA; Ly-52; Lymphocyte antigen 52; M1/69-J11D heat stable antigen; MGC75043; Nectadrin; R13-Ag; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; X62 heat stable antigen
Gene Aliases: CD24; CD24A; HSA; Ly-52; nectadrin
UniProt ID: (Human) P25063, (Mouse) P24807
Entrez Gene ID: (Human) 100133941, (Mouse) 12484
response to hypoxia
cell activation
regulation of cytokine-mediated signaling pathway
response to molecule of bacterial origin
pre-B cell differentiation
leukocyte migration involved in inflammatory response
positive regulation of T cell mediated immune response to tumor cell
positive regulation of inflammatory response to antigenic stimulus
positive regulation of B cell apoptotic process
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell surface receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
neuromuscular synaptic transmission
negative regulation of neuroblast proliferation
apoptotic mitochondrial changes
positive regulation of neuron projection development
cell migration
regulation of cell-cell adhesion
positive regulation of cell-cell adhesion
apoptotic nuclear changes
negative regulation of B cell proliferation
neuron projection development
T cell costimulation
chemokine receptor transport out of membrane raft
negative regulation of transforming growth factor beta3 production
positive regulation of integrin activation
positive regulation of cell adhesion mediated by integrin
regulation of cell-cell adhesion mediated by integrin
positive regulation of cell-cell adhesion mediated by integrin
negative regulation of erythrocyte clearance
homotypic cell-cell adhesion
negative regulation of erythrocyte aggregation
positive regulation of T cell homeostatic proliferation
positive regulation of activated T cell proliferation
regulation of phosphorylation
cholesterol homeostasis
positive regulation of MAP kinase activity
regulation of MAPK cascade
response to estrogen
regulation of B cell differentiation
negative regulation of neuron differentiation
respiratory burst
negative regulation of T cell homeostatic proliferation
positive regulation of alpha-beta T cell proliferation
synaptic vesicle endocytosis
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of neurogenesis
positive regulation of calcium-mediated signaling
positive regulation of protein tyrosine kinase activity
apoptotic signaling pathway
immune response-regulating cell surface receptor signaling pathway
Wnt signaling pathway
regulation of epithelial cell differentiation
B cell receptor transport into membrane raft
glomerular visceral epithelial cell differentiation
glomerular parietal epithelial cell differentiation
intrinsic apoptotic signaling pathway
cell-cell adhesion
positive regulation of nephron tubule epithelial cell differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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