Search
Invitrogen
EpCAM Monoclonal Antibody (323/A3)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 14
EpCAM Antibody (MA5-12436) in ICC/IF
Immunofluorescence analysis of EpCAM was performed using HT-29 and BJ cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EpCAM Mouse Monoclonal Antibody (Product # MA5-12436) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A28175) at a dilution of 1:... View More
Product Details
MA5-12436
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
323/A3
Immunogen
MCF-7 human breast cancer cells
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-12436 targets Epithelial Specific Antigen in FACS, ICC/IF, IHC (P), and RIA applications and shows reactivity with Human and Mouse samples.
The MA5-12436 immunogen is mCF-7 human breast cancer cells.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Adenocarcinoma-associated antigen; adenocarcinoma-associated antigen precursor (KSA); CD326; Cell surface glycoprotein Trop-1; EGP; EGP314; Ep-CAM; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; epithelial glycoprotein (EGP) precursor; Epithelial glycoprotein 314; hEGP314; human epithelial glycoprotein-2; KS 1/4 antigen; KSA; lymphocyte antigen 74; Major gastrointestinal tumor-associated protein GA733-2; mEGP314; membrane component, chromosome 4, surface marker (35kD glycoprotein); panepithelial glycoprotein 314; Protein 289A; TACSTD1; Trop-1 protein; trophoblast cell surface antigen 1; Tumor-associated calcium signal transducer 1; unnamed protein product
Gene Aliases: Ber-Ep4; BerEp4; CD326; DIAR5; EGP; EGP-2; EGP314; EGP40; Ep-CAM; EPCAM; EpCAM1; ESA; GA733-2; gp40; HNPCC8; KS1/4; KSA; Ly74; LYNCH8; M1S2; M4S1; MIC18; MK-1; MOC-31; Tacsd1; TACSTD1; TROP1
UniProt ID: (Human) P16422, (Mouse) Q99JW5
Entrez Gene ID: (Human) 4072, (Mouse) 17075
positive regulation of cell proliferation
signal transduction involved in regulation of gene expression
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
stem cell differentiation
cell-cell adhesion
negative regulation of cell-cell adhesion mediated by cadherin
positive regulation of cell motility
positive regulation of stem cell proliferation
ureteric bud development
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support