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Invitrogen
HK2 Monoclonal Antibody (H.738.7)
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FIGURE: 1 / 6
HK2 Antibody (MA5-14849) in WB
Knockout of HK2 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR1034965_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of HK2 was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHeLa HK2 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlo... View More
Product Details
MA5-14849
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
H.738.7
Immunogen
Synthetic peptide corresponding to residues surrounding Ser340 of sequence of human hexokinase II.
Conjugate
Form
Liquid
Concentration
24 µg/mL
Amount
2.4 µg
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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