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Invitrogen
MNK1 Monoclonal Antibody (E.611.4)
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FIGURE: 1 / 3
MNK1 Antibody (MA5-14928) in WB
Western blot was performed using MNK1 Monoclonal Antibody (E.611.4) (Product # MA5-14928) was performed and a 51 kDa band corresponding to MNK1 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), Hep G2 (Lane 2), HEK 293 (Lane 3), HeLa (Lane 4), Neuro 2a (Lane 5), C2C12 (Lane 6), U2-OS (Lane 7), Mouse Lung (Lane 8) Mouse Kidney (Lane 9) and Mouse Heart (Lane 10) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto ... View More
Product Details
MA5-14928
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
E.611.4
Immunogen
Synthetic peptide corresponding to the sequence of human Mnk1
Conjugate
Form
Liquid
Concentration
169 µg/mL
Amount
16.9 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
The Map kinase-interacting kinases (MKNK1 and MKNK2) are activated by Map kinases, regulate protein synthesis by phosphroylation eIF4E on Ser209, and may contribute to tumorigenesis. Overexpression of Mnk2 results in increased phosphorylation of endogenous eIF-4E, showing that it can act as an eIF-4E kinase in vivo. Mnk2 may play a role in the response to environmental stress and cytokines. Expression of active mutants of MNK1 and MNK2 in 293 cells diminishes cap-dependent translation relative to cap-independent translation in a transient reporter assay. Human Mnk2 is homologous to murine Mnk2 (approximately 94% identical) and human Mnk1 (71% identical). In vitro phosphorylation studies show that Mnk2 is a significantly better substrate than Mnk1 for extracellular signal-regulated kinase 2 (Erk2), p38MAPKalpha, and p38MAPKbeta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MAP kinase interacting serine/threonine kinase 1; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine-protein kinase 1; MAPK signal-integrating kinase 1; MAPK-interacting protein kinase-like kinase 1b; OTTHUMP00000009675; OTTHUMP00000009676; unnamed protein product
Gene Aliases: 2410048M24Rik; MKNK1; MNK1
UniProt ID: (Human) Q9BUB5, (Mouse) O08605
Entrez Gene ID: (Human) 8569, (Mouse) 17346
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
calmodulin-dependent protein kinase activity
protein binding
calmodulin binding
ATP binding
calcium-dependent protein serine/threonine kinase activity
kinase activity
transferase activity
metal ion binding
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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